We narrowed to 487 results for: multi

...barcodes infect the target cell population at low multiplicity of infection (MOI) such that each individual...erlotinib. Looking at barcode abundances among multiple replicate populations, they found that similar...went on to show that combination treatment with multiple drugs reveals even smaller preexisting populations...

...ELISA is best used to detect proteins for which multiple antibodies that recognize different epitopes are...sensitive to the target protein due to the use of multiple antibodies. Figure 1: Overview of using...primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can bind to a single primary...

...achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ...widely successful in primates. The authors used a multispecies approach to generate and screen a library of... Multiple Authors...

... of time that you want to probe your blot with multiple antibodies. You’ll want to set up your blot with...stripping methods. If you are planning to detect multiple proteins through several rounds of stripping and...clearing the way for the second blot. There are multiple ways to strip blots, and the best choice will ...

...ways to deal with this issue. One way is to grow multiple bacterial cultures of your AAV transfer plasmid...aliquots to use for qPCR titering. This helps avoid multiple freeze/thaws of your larger aliquots of virus.... a few different concentrations of virus, or multiplicity of infections (MOIs), to determine the optimal...

...Clustal is our frequent choice for pairwise or multiple sequence alignments of nucleotide or protein sequences... sequences. We also use COBALT for aligning multiple protein sequences, particularly for comparing different...//www.ebi.ac.uk/Tools/webservices/#multiple_sequence_alignment_msa http://www.ebi.ac.uk/Tools/webservices...

...exponential decay process we observed, while 𝜓z(t) multiplies the likelihood by the corresponding factor 𝜙...parameter. We approximated the data likelihood with multivariate Gauss-Hermite quadrature, since the analytical...intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects of weekends...

...powerful imaging technologies, the combination of multiple fluorescent protein sequences to develop diverse...individual plasmids and are each flanked by the same multiple cloning site (MCS). Modules are assembled one ...not suited to generate polycistronic containing multiple identical polyadenylation signals or promoters...

...libraries utilize an engineered Cas9, which contains multiple point mutations in the nuclease domain. These ...affected by gene activation rather than depletion. Multiple strategies have been developed to recruit transcriptional...domain such as VP16 to dCas9 or the recruitment of multiple copies of VP16 termed the “Sun Tag” method (Tanenbaum...

...Feng Zhang's group previously packaged Cas9 and multiple gRNAs into separate AAV vectors, increasing overall... AHM, Zhu K, Wagers AJ, Church GM (2016) A multifunctional AAV–CRISPR–Cas9 and its host response. Nat ... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...

... not, multiple techniques are used as part of a treatment plan, an approach known as multimodal therapy...are some genetic factors involved. For example, multiple studies show that certain mutations of the BAP1...

...different viral families and can be used to target multiple delivery systems, such as lentivirus and MMLV ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi... Multiple Authors...

...individual genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the... then packaged into AAV for in vivo delivery. Multiplex gene targeting is also possible and is only constrained...vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator...

...polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

...CRBNmidi alone (A) and in a ternary complex with the multiple myeloma drug mezigdomide and ubiquitination substrate...mutations.  Here, the researchers were able to target multiple genes in M. abscessus either simultaneously or... Multiple Authors...

...maintain itself within host cells.  The plasmid has multiple ori that are functionally differentiated from ...proteins from bacterial hosts. In a plasmid with multiple oris, one may function in one type of host and...only one ori and a broad host range plasmid with multiple oris that vary in DnaA-box and iteron structure...

...et al., 2019). Because of its ability to target multiple gene loci simultaneously, CRISPRa can also be ...(Chavez et al., 2015)) are good choices across multiple cell lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3...Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods 13:563–567 . https://doi....

...with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although this ...for lactate developed using a high-throughput multiparameter screen (Koveal et al. 2022). Lactate is a key... here!   Koveal D, et al. A high-throughput multiparameter screen for accelerated development and optimization...

...chromosomal location, sequence context, and cell type. Multiple labs worked to improve its effectiveness through... a machine learning approach, the lab proposed multiple sets of Cas9 mutations that conferred new PAM ...Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science...

...calculate things about earlier ones. In practice, multiple sources of error (red) influence these relationships...not perturbed by the experiment Unfortunately, multiple reports suggest that common housekeeping proteins... to worry about the linear detection ranges of multiple targets. Convenient! Comparison of loading control...