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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 363 results for: nsf

Showing: 161 - 180 of 363 results

  1. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...of University of Michigan says, “Addgene has transformed the way in which we share plasmids and other ...shipping logistics and facilitate all material transfer agreements, thereby freeing up time for our depositors...

  2. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...includes an antibiotic resistance marker. After transformation, plate the cells on medium containing the corresponding...selection will select for cells that have been transformed with the plasmid backbone (with or without your...

  3. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...encompasses millions of choices including pharma, tech transfer, management consulting, science communication,...where you want to go will help you focus on the transferable skills that you will need to get there. Additional...

  4. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...

  7. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...However, careers outside of the lab highlight “transferable” skills such as leadership, management, and ...Leadership Networking University technology transfer office positions   Market analyses Patent...

  8. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... like gel electrophoresis to separate DNA or transforming cells with green fluorescent protein, these ... into a host cell (through a process called transformation), essentially giving the cell all the instructions...

  10. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...to modify it to improve yields. For example, transfecting the producer cell line with an expression vector...PMC3345986. Meliani, Amine, et al. "Enhanced liver gene transfer and evasion of preexisting humoral immunity with...

  11. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...E. coli to maintain the final plasmid after transformation. Addgene depositors Sylvestre Marillonnet and...Parts kit contains modules specific for plant transformation. Weber et al., PLoS One. 2011. Feb 18;6(2...cloning system for the rapid assembly of plant transformation constructs. As the name suggests, GreenGate...

  13. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...supplied with a luciferase substrate, the luciferase transfers energy to the FP, resulting in a fluorescent signal... principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase...

  14. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Animal Models Q9: Is there any difference in transfection efficiency when delivering these viruses via...cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced CNS tropism to other serotypes...

  15. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ... and the nonmethylated PCR products are then transformed into E. coli to undergo nick repair. This method...template plasmid, these PCR products are directly transformed into bacteria expressing recombineering proteins...

  16. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ... genetic system, and a highly versatile DNA transformation system.Unlike most other microorganisms, yeast...plasmids oftentimes utilize an ARS to aid in high transformation efficiency; however, this region does not necessarily...

  17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...gene in frame with an MCS. E. coli that were transformed with the empty vectors expressed the ccdB gene... that ccdB Survival™ can be more difficult to transform, depending upon the specific plasmids being used...

  19. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...Part of that work meant facilitating the Material Transfer Agreement process, which was a major barrier for... for depositors and requestors alike. We transformed this process by aligning institutions with standardized...

  20. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ... microbial species. These also contain the RP4 transfer origin as well as a selectable marker and a desired...Gilliamella apicoli. The plasmids could also be transformed into Alphaproteobacteira, Betaproteobacteria,...
Showing: 161 - 180 of 363 results