We narrowed to 230 results for: pET

1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

   Type

   Blog Post

   Published

   March 8, 2017, 3:15 p.m.

   ...cloning strain DH5α. For plasmids with highly repetitive sequences (prone to recombination in bacteria...

2. Hot Plasmids and Viral Preps - January 2021

   Type

   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ...cells. Casɸ from Biggiephage can eliminate competing mobile genetic elements. Image by Basem Al-Shayeb...

3. Meet an Addgenie: Insights into Grad School, Career Paths, and More

   Type

   Blog Post

   Published

   June 25, 2020, 1:15 p.m.

   ...discovered that science here is more stressful and competitive than back home. Scientists are often under a ...

4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...

5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...

6. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...validation experiments performed will be useful in perpetuity.  Second, plasmids are easy to store and share...

7. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ... platform, such as GC-rich regions and highly repetitive sequences and/or features. Most of these regions...

8. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...Hurt for his help on this piece. Rob is postdoc in Peter Schultz’s group at Scripps Research, where he works...

9. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ... This blog post was originally written by Maya Peters Kostman in May 2020 and updated by Jason Nasse ...

10. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ... Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors ...

11. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ... weaker poly(A) signals like BGH.  References Peters JM, Vangeloff AD, Landick R. Bacterial Transcription...

12. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...pathways are involved in RNA based surveillance of repetitive gene families and provide a way to guide inheritance...

13. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...Postdoctoral Association (NPA) outlines 6 Core Competencies that all postdocs (but also PhDs) should develop...

14. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ...attend conferences makes it very challenging to compete in today’s tough academic or industry job market...

15. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...bacterial strains, like NEB Stable. NEB Stable competent cells lack RecA, a protein that aids in the homologous...

16. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    Published

    April 26, 2022, 1:15 p.m.

    ... RQ value of 1.5 means you have either made a pipetting error or an incredible discovery. The range of...

17. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...References Loeff, L., Brouns, S. J., & Joo, C. (2018). Repetitive DNA reeling by the Cascade-Cas3 complex in nucleotide...

18. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ... is easier, less invasive, and only requires a pipette. Since the substances are administered through ...

19. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    .... M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction...

20. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    Published

    Aug. 4, 2016, 2:30 p.m.

    ...blogs, or in the comments section below. Keep pipetting and happy genome editing! Check Out Other Argonaute...