We narrowed to 461 results for: pac

...in mind, but likely will not have a significant impact on your data. You are trying to generate a knockout...depending on your platform, off-target effects should impact your experimental design differently. Yes, it is...optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few...

... professional capacity generated during this process result directly in positive impacts on the lives ... a level of sympathy that is difficult to find. Impact of the International Mentorship Program and the...

...between the transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...

... a bit like a Fedex delivery. Your fluorophore package needs to go to the proper mailbox (i.e., a binding...that can nonspecifically soak up your fluorophore package. One important concept in FRET experimental design...synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1.tR4-MbPyl pyrrolysyl-tRNA synthetase Mammalian ...

... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...CRISPRi screen to identify endogenous genes that impact CGBE efficiency. In addition to using a new UNG...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...

... year to give our lab and shipping teams enough space at HQ and for some of us, that means spending more...co-workers” have made their way onto our Slack workspace over the last year. Now, we’ve collected photos...

...week to celebrate the scientists who make a big impact in open science. Depositors consistently contribute...depositor fun facts for a quick way to grasp the big impacts depositors have on science globally. *All data ...

...titering. Our newest viral vector service, AAV Packaged on Request  has seen a fantastic response from...our in-stock viral vectors or consider our AAV Packaged on Request service, which offers over 3,000 eligible...

...to younger graduate students   Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...studies developmental gene regulation with Dr. Angela DePace in the Department of Systems Biology, and he tweets...

...and moved to our packing area.Using the barcode system, the correct plasmids are packed into their boxes...

...Running your transfer in the cold room or packing the box with ice packs can help keep the system cool, as can...overheating. Try running it in a cold room or putting ice packs in and/or around the gel box. If overheating remains...

...the talent pipeline sets the world up for reduced impact of scientists and science. The pandemic has demonstrated...of priorities for pd|hub which include building capacity to identify the most effective practices and disseminating...

...yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful for producing...difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins and may recapitulate the...

...relatively the same for quite a while and hadn’t kept pace with our growth over the last few years. Some of...elements and allowed our content to dictate the space. We wanted to comfortably tell our story, to get...

... consider. To tease apart how different neurons impact a certain output, neuroscientists have begun to...improve delivery by increasing transgene cargo capacity, enhancing anterograde transport efficiency, and...

...role in fighting the pandemic could be even more impactful by reaching more scientists. Many members of the...past months, we’re excited to see our open sharing impact grow beyond COVID-19. But, we can’t do this alone...

...variety of metrics they can use to evaluate the impact of their work (H-index for instance), but these...the Addgene Blog Read How Notions of Scientific Impact Are Evolving Check out the Blog Post on Our Citations...

...4D nucleome, the organization of the nucleus in space and time, and to understanding how nuclear organization...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

...circulating tumor cells (CTCs) is going to have a big impact on our understanding and treatment of cancer. It... I hope that our antibody tentacles may have an impact on this challenge either for magnetic capture or...

...add the citation and release the hold. Increasing impact Making the plasmids available as soon as your paper...paper is published helps maximize the potential impact of your work. Plus, it’s costly for us to process...