We narrowed to 325 results for: pho;
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TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....costs low. Most will work with you remotely (by phone, Skype or Google Hangout) but in-person coaching...White Consulting frequently do sessions by Skype or phone. Samantha Sutton is another very experienced and...
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Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....actions of the whole complex. For example, the phosphorylation status of SRC-3 defines its association with... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....contact information, and potentially a photograph of yourself. A photo can be very useful in the event that... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m....frequent CDG type characterized by deficient phosphomannomutase (PMM) activity due to mutations in the PMM2... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones). Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m.... of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system...for clearer characterization of the location, morphology, and circuit mapping of a targeted neuronal population... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....taking a snapshot of chromatin looping with the photo “developed” by analyzing DNA sequencing data. While... The high resolution and multiplexed chromatin ‘photos’ generated with CAPTURE 2.0 allow us to uncover... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....commonly used promoters include the phosphate and copper inducible PHO5 and CUP1 promoters respectively. ... -
Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....Screening Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Libraries ~11,000–110,139 ... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate... Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed cell... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....your samples and maintaining cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker...reagents - cross-linkers better preserve sample morphology, while organic solvents can reveal otherwise ... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....The kan resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....basic science research (neuronal tracing and morphologic studies), as well as translational applications...capsids are useful tools for neuronal tracing and morphology studies when used as part of a two part fluorescent... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....Using a spectrophotometer is among the faster methods of quantification. Many spectrophotometers have an... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m.... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....recently purified MDH to study the impact of phosphorylation on the enzyme’s structure, function, and interaction... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....including a lot of future Addgenies as shown in the photo below. Kids and adults alike enjoyed some friendly...of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget about... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....this unique sequence of DNA and both identified lymphocyte antigen 6 complex (LY6A) as a receptor for PHP.B...discovered as an antigen upregulated on activated lymphocytes and is commonly used to enrich adult mouse hematopoietic... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....have diverse traits with good genetic mapping morphology and interesting behaviors. There is diversity...Chisholm) This cyanobacterium is the smallest photosynthetic cell in existence, but most abundant cell on...