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We narrowed to 359 results for: plasmids 101

Showing: 161 - 180 of 359 results

  1. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...be found in our Plasmids 101 blog series. Want to learn more about sharing your plasmids via Addgene? Check... resources, such as protocols, Plasmids 101 articles on various plasmid features, and reference tables... requested plasmids. I wondered how many scientists have used the protocol for this plasmid, and if it... your protocols onto your plasmid page, scientists who request your plasmid will have easy access to your...restriction digest of plasmid DNA, and bacterial transformation. And more plasmid-related educational resources...deposit their plasmids to the repository. These protocols show up on the related plasmid pages and can...http://www.addgene.org/ If you’re working with plasmids and you decide to share them using Addgene’s repository...

  2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ...interest has been cloned into an expression plasmid, the plasmid can be introduced into host cells, such ...that the plasmids and sequences for antibodies are not shared. For instance, through open plasmid sharing...Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: What...Recombinant monoclonal antibodies are encoded in plasmids and produce a single antibody recognizing one ...synthetic genes that are typically expressed from a plasmid or from an integrated sequence in a stable cell...heavy chain genes of the antibody are encoded in a plasmid. (Right) An assembled antibody protein binds an...laboratories and companies have already begun creating plasmids encoding affinity reagents. These include conventional...

  3. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...inconspicuous coloring.  FLIS plasmid Recently, the Phuels lab deposited a plasmid that will forever change...blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based system...

  4. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ... after ordering a plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your...with 210 plasmids from the collection. You can find the full list here. Most of these plasmids are empty...the Addgene blog Browse blog posts from our Plasmids 101 blog series Learn about the different grades...cloning experiment? What if after receiving the plasmid from Addgene you could directly start your digest...you can focus on what you want to do with your plasmid without spending time and resources in preparative...research and discovery.” Here are the details. Plasmids offered as cloning grade DNA As we begin testing...molecular cloning. Plus, we decided to include 10 plasmids that can be directly used in the research on the...

  5. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org ...

  7. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...lentiviral plasmids include popular envelope and packaging plasmids as well as transfer plasmids. Download...cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed a break so they're hanging...Otherwise, check out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can... the plasmids that Addgene offers by visiting our collections page. Here, you can browse plasmids by field...your favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re...coronavirus collection page, where you can find plasmid tools to accelerate your COVID-19 research.  Download...background.         If you’re predominantly a plasmid user, instead, don’t fret; we have backgrounds ...

  8. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org...these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...protein fusions Browse the fluorescent proteins plasmid kits ...

  9. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...Addgene blog Browse blog posts on plasmid cloning Read our Plasmids 101 blog posts Learn about the differences...Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important... into a plasmid backbone. Transfection grade DNA Figure 3: Need to introduce plasmids into mammalian... an external plasmid in mammalian cells and it will likely require micrograms of plasmid DNA for each ...utilize your plasmid DNA, so that you can decide what will be the optimal type of plasmid preparation ...purity. Based on these premises we can classify a plasmid preparation in 3 different ways: Transformation...Transformation introduces transformation grade plasmids into a bacterial cell. Transformation grade...

  10. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ... helps scientists understand how to use CRISPR plasmids in the lab and the vast range of possibilities...resources on the Addgene blog PRIME Editing CRISPR 101: Cas9 vs the Other Cas(s) Progress Towards a PAM-...

  11. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ... tag expressed on a plasmid, allowing for in vivo biotinylation through a plasmid-based biotin ligase....the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...which can easily be cloned into a gRNA expression plasmid. Experimental use Since the initial report describing...currently no cure (Campbell et al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated... PubMed PMID: 24201379. PubMed Central PMCID: PMC3821016. Fujita, Toshitsugu, et al. "Identification of...

  12. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our...to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water?... We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors who quickly shared their ...you're thinking about hosting multiple plasmids in one cell, it might be that you can't stably maintain...This blog post features two mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without...CRISPR to detect SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without...

  13. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook includes the...commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease... to deliver. Not all cells efficiently take up plasmids used to produce CRISPR systems    ...

  15. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101 Biotinylation Other resources...vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and... specific locations in the cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate peroxidase... They named this mutant APEX2.  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity...allowing for more precise temporal control.  Find plasmids for TurboID and miniturbo Split-BioID: A conditional...streptavidin and separated via flow cytometry.  Find plasmids for EXCELL here! Since BioID was developed in ... PubMed PMID: 24114984. PubMed Central PMCID: PMC3910158. Ramanathan, Muthukumar, et al. "RNA–protein ...

  16. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... Organisms blog post Read more about plasmids in our Plasmids 101 blog series Learn more about CRISPR ...box” for its common use in plasmid manipulation and genetics research. Plasmids are ideal tools for studying...editing in our CRISPR 101 blog series Resources on Addgene.org: Browse our plasmid collection page Find...Find more plasmids and resources for plant resources on Plant Plasmids Collection Page Read our molecular...disease models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus genes! Mexican axolotl...several vectors for use in the axolotl including plasmids for use in CRISPR and Tol2 transposase induced... and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes and a tool-kit from...

  17. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series Learn...Reference Read about genetic code expansion Browse our plasmid collection page  ... in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech.2004.04.006 Hausser J, Syed AP, Bilen.... Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112 Mauro VP, Chappell SA (2014) A critical...Molecular Medicine 20:604–613  https://doi.org/10.1016/j.molmed.2014.09.003 Nakamura Y (2000) Codon usage...Neuroscience Methods 124:75–81 . https://doi.org/10.1016/s0165-0270(02)00362-x Stadler M, Fire A (2011) ...USA 107:3645–3650 https://doi.org/10.1073/pnas.0909910107 Varani G, McClain WH (2000) The G·U wobble base...

  18. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ... kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on Addgene.org...fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during plasmid DNA ...Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...Nowadays, the most common E. coli strains used for plasmid amplification are endA negative, meaning the gene...degradation and which experiments you can use your plasmid.  DNA elution choices: TE, Tris buffer, or water...polymerases. In the situation where you need to use plasmid in TE for any enzymatic reaction, an effective ...choices that companies offer for the elution of plasmid DNA through their kit. The advantage of using a...

  19. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant ... recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...binding protein. Cell 46:291–300. https://doi.org/10.1016/0092-8674(86)90746-4 Munro S, Pelham HRB (1987)... ER proteins. Cell 48:899–907. https://doi.org/10.1016/0092-8674(87)90086-9  Schüchner S, Behm C, Mudrak...

  20. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse our plasmid collection... resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of mice as model... pancreas. They first tagged these cells using plasmids. Then using HOTcre, a method that uses heat induction... feeding the worm bacteria expressing specific plasmids (L4440) that contain your gene of interest (Fire...calcium signaling in the entire adult brain, using a plasmid that expresses a genetically encoded calcium sensor...al., 2015). If you are interested in C. elegans plasmids and resources check out Addgene’s Worm Expression...pages Read our molecular biology reference Find plasmids for your research ...
Showing: 161 - 180 of 359 results