We narrowed to 225 results for: poly

1. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ...whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic...

2. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   Published

   July 19, 2018, 1:12 p.m.

   ...broader impacts for their work in education and policy and to draw attention and support to work in their...

3. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they were at the...

4. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   Published

   Feb. 2, 2021, 2:15 p.m.

   ...previously mentioned, enlist the help of your network to polish the final draft prior to submission. When in doubt...

5. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...

6. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are having difficulties...

7. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

8. Summer SciComm: Preprints

   Type

   Blog Post

   Published

   Aug. 16, 2022, 1:15 p.m.

   ...comment online! Want the general public (say, policymakers or community organizations) to use your work...

9. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...

10. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

11. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

12. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier...

13. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    Published

    July 18, 2019, 12:55 p.m.

    ... C. elegans 3’UTRs structures, alternative polyadenylation for all protein coding mRNAs, and annotations...

14. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...

16. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...

17. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    Published

    Oct. 22, 2014, 1:57 p.m.

    ...want to take over? It is the moderator's job to politely but firmly get them to stop talking and redirect...

18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows...

19. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...