We narrowed to 314 results for: reporter

1. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

   Type

   Blog Post

   Published

   Jan. 8, 2014, 3:10 p.m.

   ...added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA for use ...

2. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   Published

   July 12, 2016, 2:30 p.m.

   ... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... R (2013) One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated...

3. Reflections and Looking Ahead

   Type

   Blog Post

   Published

   Feb. 25, 2025, 2:15 p.m.

   ...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...

4. New Viral Vectors - Fall 2024

   Type

   Blog Post

   Published

   Oct. 3, 2024, 1:15 p.m.

   ...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

5. Cellosaurus: A Cell Line Information Database

   Type

   Blog Post

   Published

   Feb. 21, 2023, 2:15 p.m.

   .... For instance, recently Cellosaurus received a report that HT-55, which was described as a colon cancer...

6. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

   Type

   Blog Post

   Published

   Jan. 21, 2020, 2:30 p.m.

   ...also captured as metadata.   Screen results are reported as the original quantitative gene-level scores...

7. SunTag and Fluorescent Imaging

   Type

   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ...protein function, thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum...

8. pSiM24: Simplifying Plant Genetic Engineering

   Type

   Blog Post

   Published

   Sept. 29, 2015, 2:30 p.m.

   ... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ...

9. Seeing Red: Simple GFP Photoconversion

   Type

   Blog Post

   Published

   Aug. 4, 2015, 2:30 p.m.

   ...conduct subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs) are also...

10. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type

    Blog Post

    Published

    Feb. 18, 2015, 3:09 p.m.

    ...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...

11. Future of Research Conference - Remarkable Opening Session

    Type

    Blog Post

    Published

    Oct. 7, 2014, 3 p.m.

    ...for PIs to include in their annual NIH progress reports information on the Individual Development Plans...

12. 9 Tips to Achieve Success in Academia

    Type

    Blog Post

    Published

    April 1, 2014, 5:33 p.m.

    ... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...

13. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing...recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed PMID: 29743652. PubMed...

14. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ...piggyBac plasmids    Tol2  Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ...

15. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ...

16. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by...

17. New Viral Vectors - Winter 2025

    Type

    Blog Post

    Published

    Jan. 28, 2025, noon

    ...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

18. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type

    Blog Post

    Published

    Aug. 11, 2020, 1:15 p.m.

    ...’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such...

19. Hot Plasmids and Viral Preps - July 2021

    Type

    Blog Post

    Published

    July 27, 2021, 1:15 p.m.

    ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible...

20. Summer SciComm Series: Master of SciComm

    Type

    Blog Post

    Published

    July 21, 2022, 1:15 p.m.

    ...itself. Several years of exclusively writing lab reports and empirical essays had also stirred some of my...