We narrowed to 308 results for: reporter
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TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m....conduct subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs) are also...
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CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m.... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also... -
New Viral Vectors - Fall 2024
TypeBlog PostPublishedOct. 3, 2024, 1:15 p.m....check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link... -
Cellosaurus: A Cell Line Information Database
TypeBlog PostPublishedFeb. 21, 2023, 2:15 p.m..... For instance, recently Cellosaurus received a report that HT-55, which was described as a colon cancer... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog PostPublishedJan. 21, 2020, 2:30 p.m....also captured as metadata. Screen results are reported as the original quantitative gene-level scores... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m.... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m.... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m.... targeting astroglia. It has additionally been reported to have tropism for lung, liver, vascular endothelial...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....piggyBac plasmids Tol2 Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing...recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed PMID: 29743652. PubMed... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m....with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal electrical activity... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....following sucrose treatment. Moreover, several reports have shown that cells treated with sucrose or other... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and genome engineering... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....itself. Several years of exclusively writing lab reports and empirical essays had also stirred some of my... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m.... rather than to rely on nuclei extraction. They report that this change offers a significant improvement...