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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 430 results for: sequence

Showing: 161 - 180 of 430 results

  1. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...The amplified library is verified through NGS sequencing to confirm library representation. Transfection...then be used for genetic screening, followed by sequencing and further data analysis. Based on Figure 31.5.2...need to verify your library by next-generation sequencing (NGS). The representation of your amplified library...each library’s page, you can find protocols for sequencing, representative NGS results (read counts or another...whether selecting colonies and performing Sanger sequencing will suffice…and the answer is no. Due to the...

  2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ... MazF is a ribonuclease that cleaves specific sequences in RNA resulting in a blockage of protein synthesis...endonucleases cleave DNA at or near specific nucleotide sequences. To counter this, methyltransferases add methyl...methyl groups to bases at specific nucleotide sequences, preventing the cognate restriction enzyme from cleaving...coupled. Translation from the Shine-Dalgarno (SD) sequence of mok results in translation of Mok and Hok, ... death. However, the sok antisense-RNA binds a sequence downstream of the mok SD resulting in a double...

  3. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...eukaryotic cells.1,2 Since then, a variety of IRES sequences have been discovered - many from viruses, but ...leading to separation between the end of the 2A sequence and the next peptide downstream.4 The "cleavage...experimental conditions.   Peptide  Amino acid sequence* T2A: (GSG) E G R G S L L T C G D V E E N P ...of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Pelletier et al (...Multicistronic Lentiviral Vectorswith Peptide 2A Sequences. Ibrahimi et al. (Hum Gene Ther. 2009 Aug;20(8...

  4. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...walkthrough of how you can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie... generate C->T or G->A transitions in a target sequence in live cells. This technique uses catalytically...a deaminase to make the transitions at target sequences specified by gRNAs. Caveats, potential specificity...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays these...these requirements in regard to their sequence (NGG, etc.) as well as their orientation relative to the...Adler et al.   You can also search by protein sequence with BLAST, using the homepage search bar. Once... other parameters, such as E-value for protein sequence searches. Additionally, the Phylogeny tool allows...

  7. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... as a guide to complementary RNA sequence. Once a compatible sequence is found (typically RNA that the...purposes similar to that of vaccines. If you know the sequence of a virus, you could introduce some of its dsRNA...due to their abundance of R-loops and repetitive sequence. All of the R-loops described above play distinct...productive levels of R-loops and putting DNA at risk. Consequences of duplexed RNA Mammalian cells have multiple...sensing systems fail, diseases and steep cellular consequences arise. dsRNA An immune response is activated...Cellular origins of dsRNA, their recognition and consequences. Nat Rev Mol Cell Biol, 23: 286-301 (2022). ...

  8. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...whole exome sequencing of embryo derived-ES cells.They also conducted whole genome sequencing in selected...rates to untreated controls. Ma et al. performed sequencing of 23 potential off-target editing sites in the...off-target effects, they limited their whole genome sequencing to a small percentage of edited embryos. Since...

  9. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...with a 1.9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More...libraries and can be combined with high-throughput sequencing to perform multiplexed analysis of cells pre-...backbones) here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used...

  10. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...Yamagata et al used previously published nanobody sequences that targeted the following antigens: GFP, RFP...actin-binding protein gelsolin, but any nanobody sequence could be used to make a RANbody. RANbodies are...secreted from cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell culture...useful RANbody, consider leaving a link to its sequence in the comments below. References Sanes, J.R....

  11. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...phosphate backbone modifications The recognition sequence for CRISPR-Cas9 targeting is led by a ~20 bp gRNA...CRISPR RNA (crRNA). In addition to the 20 bp guide sequence, the crRNA also contains a ~20 bp repeat region...validated gRNA repository to find pre-tested gRNA sequences for your next CRISPR experiment.    Chemical...which is photocaged and unable to engage a target sequence. Upon a brief light exposure (several seconds)...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...easy enough to contemplate DNA in terms of its sequencing, in vivo studies should always take DNA’s chromatin...expression pattern and an auxin inducible degron (AID) sequence attached to your protein of interest. TIR1 interacts...of auxin, this TIR1 complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged...(minimal 44 amino acids auxin inducible degron sequence) tagged proteins. Degradation of your protein ...

  13. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...adding long-read sequencing capabilities to complement our next-generation sequencing, setting new standards... We’re also building a portal to easily share sequences and characterization data for over 150,000 plasmids...

  14. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...containing the modifying sequence flanked by approximately 50 base pairs of sequence homologous to the chromosomal... typically contain both a protein modification sequence and a selectable gene product for isolation of...

  15. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... reaction for traditional restriction cloning. Sequence and Ligation Independent Cloning (SLIC) could ...Invitrogen and based on lambda phage integration sequences, relies on site-specific recombination reactions...components and define their final order through sequence homology. Similarly, Golden Gate assembly allows...FastCloning: a highly simplified, purification-free, sequence-and ligation-independent PCR cloning method." ...

  16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... the exact original sequence, it can restore the original protein-coding sequence using a recoding strategy...synthetic sequence will only spread in transgenic laboratory populations with that sequence2. Both methods...; a subsequent drive can restore the wild-type sequence (save for the residual sgRNAs and possibly cas9...unpredictable ecological effects. Because the consequences of such a mistake would necessarily extend far...

  17. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method for mapping where...noise, reducing the read depth necessary when sequencing and lowering the amount of tissue needed per ...subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN method and using...

  18. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...Transposons are sequences of DNA that can move around in a genome. In a laboratory setting, transposons...the tranposon to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion...between a greater number of extinct transposon sequences and then testing various combinations of changes...

  19. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...?  scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of hybridomas...semi-synthetic or entirely synthetic VL and VH sequences. Phage display also allows for screening scFv ...antibodies. To do this, antibody variable domain sequences are expressed as scFvs and screened with in vitro...assays to select for strong binders. Then the sequences for the variable domains of the selected scFvs...

  20. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...The raw data is then normalized to a reference sequence present in the same amount in all samples (usually...to run multiplex assays, allowing the reference sequence and the gene of interest to be amplified in the...for both the gene of interest and the reference sequence. At the end of the run, each droplet is read as...Complexity of the gene of interest Some gene sequences are harder to amplify and quantify than others...
Showing: 161 - 180 of 430 results