..., developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene...Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested...Montero Llopis, P., Haining, W. N., Doench, J. G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible..., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H. (2019). PTPN2 regulates the generation of..., Coxe, D. J., Doench, J. G., Haining, W. N., & Sharpe, A. H. (2019). A CRISPR-Cas9 delivery system for...

...engineering, Dr. Jennifer Doudna and Dr. Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc...and Joan Steitz. This award ceremony honored Charpentier and Doudna, who talked about their research leading...simpler 1 protein - 1 RNA system Drs. Dounda and Charpentier met at a 2011 conference and decided to collaborate...was a simpler 1 protein - 1 RNA system.  Dr. Charpentier advocated for the crucial role of basic research...

..., developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve T cells....Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested..., Hacohen, N., Doench, J. G., LaFleur, M. W., & Sharpe, A. H. (2024). Framework for in vivo T cell screens...

...experiments involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common... yourself via needlestick or cut. Following all sharps procedures for your organization will significantly...

...science.adi1910 Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014). Classification and...Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., Horvath, P., Moineau, S., Mojica... S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D., Haft, D. H., Horvath, P., Moineau...

...given by Phil Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench...

...denaturing.  Designed ankyrin repeat proteins, or DARPins, are genetically engineered small affinity proteins...binding proteins known as ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity...

...potentially shuttling repair down a different pathway (Charpentier et al., Nat Com).     Fig. 3: HDR events... 8(2024), (2017). 10.1038/s41467-017-01836-2 Charpentier, M., Khedher, A. H. Y., Menerot, S., Brion, A...

...frequency in a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created the...Genet 12:683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure...

...Using acrylamide instead of agarose gives much sharper bands. I compared two MOIs of gRNA transduction... fragments, run PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell...

... authors were exploring were space and robots. Warping to stars in space ships and the rise of killer ...doi.org/10.1534/genetics.111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering...

...Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex ...

...information about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo CRISPR Screens...

...PC) Chromas (PC) 2. You should see individual, sharp and evenly spaced peaks …like these…. 3. Expect...

...Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, ...

... our virus. Ed Boyden’s lab from MIT and Alla Karpova’s lab from Janelia Research Campus have provided...

...Fuchs Kai Ge Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer Alexander Stark Bas van Steensel...

...immune cells has been a gap in the field. Arlene Sharpe’s group at the Harvard Medical School developed ...

...She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources...

...She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources...