... tested by Quadros et al. When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend...pups born, with typical efficiencies of 30-60%. Quadros et al. attribute much of this variation to differences... locus may also influence efficiency. However, Quadros et al. saw success with Easi-CRISPR across three...Channabasavaiah B. Gurumurthy, Masato Ohtsuka, and Rolen M. Quadros for assistance with editing this blog post. References...PMID: 26242611 PubMed Central  PMCID: PMC4525291 Quadros, Rolen M., et al. “Easi-CRISPR: a robust method...Central PMCID: PMC5434640 Miura, Hiromi, Rolen M. Quadros, Channabasavaiah B Gurumurthy, and Masato Ohtsuka...

...conceptualize where the populations are, try drawing a quadrant over the plot that divides it into four, using...different populations by imagining (or drawing) a quadrant over the dot plot. Created with biorender.com....Biorender! While a helpful visualization tool, a quadrant will often be too clean for real-world data. Most...these populations, I suggest using the imaginary quadrant to help (Figure 5). Even though this real-world...real-world data is far messier than biorender plots, quadrants can still help me as a reader if I'm struggling...strategy from Barlow-Anacker et al., 2017, with quadrants to help visualize the different populations. Used... This flow data, from Boix et al., 2018, uses quadrants to gate and has numbers representing the percentage...

...and spread over the second quadrant. The process continues until all quadrants are used, hopefully revealing...plate, first the bacteria are inoculated over a quadrant of an agar plate. Then, a stick is dragged through...revealing single, isolated colonies in the last quadrant after incubation. The challenge is usually successful...

...Dronpa that doesn't become inactivated as quickly. Padron is a monomeric, reversible PA-FP derived from mutating... / P141L / G155S / V157G / M159Y / F190S) (7). Padron’s “positive” switching behavior is the opposite ...opposite of Dronpa; when exposed to blue light, Padron is photoconverted from a dark state, to an activated state...bright green fluorescence. When exposed to UV light Padron is inactivated. The rs-Cherries (8) are another...UV-Violet Dark/Green N/A (high) High 503/ 518 Padron Monomer Blue Dark/ Green N/A (high) Low 503/ 518...

...experimentally-specific gates. Two-dimensional staining (quadrant gating) For our last example plot, I will show...cells (CD8+) from dendritic cells (CD11b+) using a quadrant gate. Within my “lymphocyte + single cell + live... two stains in relation to each other. Using a quadrant gate, I can divide my cell population into DN,...for each stain respectively). (B) Instead of a quadrant gate, I am free to use multiple individual gates...

...itself. Many thanks to our guest blogger Subhadra Jayaraman! Subhadra Jayaraman is currently a doctoral candidate...This post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University...

...determined it is most likely adrenal in origin. (Anatomy refresher: the adrenal gland is next to the kidney...

...This post was contributed by Adriana Galvan, an associate professor at Emory University School of Medicine...Connection.     Thank you to our guest blogger! Adriana Galvan is an associate professor at Emory University...

...the size limitations of ssODNs is Easi-CRISPR (Quadros et al., 2017). Easi-CRISPR in vitro transcribes...long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (2017). PubMed.  ...

...References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base ..., Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR (2017) Improved base excision repair...

...Discosoma sp. and Katushka/mKate from Entacmaea quadricolor. With his discoveries, another part of the fluorescent...

... nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly permuted...

...using long ssDNA donors". Miura, Hiromi, Rolen M. Quadros, Channabasavaiah B. Gurumurthy, and Masato Ohtsuka...

...uncle with Parkinson's, a half-brother who is a quadriplegic due to a spinal cord injury, and numerous family...

...29707602. PubMed Central PMCID: PMC5918177. Volak, Adrienn, et al. "Virus vector-mediated genetic modification...

...Deciphering the Relationship” by our guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton ...

...References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base ...

...receptors- angiotensin II type 1 receptor and the β2 adrenoceptor. GPCRs in chemogenetics  While there’s still...

...References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base ...

...serotypes (AAV1, 8, 9) injected into the A2 noradrenergic area of the caudal NST. (A-C) Low-magnification...