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Showing: 1 - 14 of 14 results
  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...Anti-Integrin alpha L [IPI-TS2/4] Integrin alpha-L Human IgG1 Rabbit Anti-Integrin alpha L [IPI-M17/... Integrin alpha-L Mouse IgG1 Rabbit Anti-Integrin alpha L [IPI-TS1/22] Integrin alpha-L Human IgG1...Anti-Integrin alpha M [IPI-M1/70] Integrin alpha-M Mouse, Human IgG1 Rabbit Anti-Integrin alpha M [IPI-CBRM1... Integrin alpha-M Human IgG1 Rabbit Anti-Integrin alpha M [IPI-CBRM1/29] Integrin alpha-M Human IgG1...Anti-Integrin alpha M [IPI-LM2/1] Integrin alpha-M Human IgG1 Rabbit Anti-Integrin alpha 5 [IPI-MFR5...] Integrin alpha-5 Mouse IgG1 Rabbit Anti-Integrin alpha L [IPI-TS2/4] Integrin alpha-L Human IgG2a...Anti-Integrin alpha L [IPI-M17/4] Integrin alpha-L Mouse IgG2a Rat Anti-Integrin alpha M [IPI-M1/70...
  2. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    Published
    June 22, 2021, 1:15 p.m.
    ...neutralize many variants, including the B.1.1.7 (Alpha variant), B1.1.298, and B.1.429 (Epsilon), nearly as well...variant) and the B.1.351 (Beta variant) were much less effectively neutralized. The B.1.617.2 (Delta variant...of luminescence.     B.1.351 variant neutralization escape The lab saw that the B.1.351 variant was very...levels of cross-neutralization occurs in the P.1 and B.1.351 variants tested. Image from Garcia-Beltran et...happening.”  In their assay, the neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses...distinct from SARS-CoV-2 and its variants. Yet, the B.1.351 variant, which has only 9 different mutations... vaccination and after both vaccinations. For the B.1.351 variant, a single vaccination didn’t result ...
  3. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly...generated using ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons...Wasielewski, L. M., Sönmez, L., Benke, D., Weber, B., Bohacek, J., Reiner, A., … Patriarchi, T. (2023)...blocked by alpha2-AR antagonist, nLight sensors are not. Instead, they are blocked by alpha1-AR antagonist...antagonist. This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine...multiplexing in experiments, including ones that study alpha2-AR. Selectivity Norepinephrine and dopamine are...
  4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... high-throughput screen for molecules affecting alpha-synuclein oligomerisation. Eastwood T, Baker K, ...FP(11) and FP(1-10) fragments. When Protein A and B interact, the FP fragments can assemble the full structure...signal amplification. Zhou S, Feng S, Brown D, Huang B. PLoS One. 2020 Bo Huang Yellow Venus pBiFC-VN173...
  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ... workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single alpha helix) and...denaturated (left) or complexes may dissociate  (right). B) Protein samples protected from AWI by helical LEAs... McElroy, A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (...
  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal...Cre-dependent SPH construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific...
  7. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...Transcriptional repression DNA Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9...Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, Klasić M, Zoldoš V (2016) Repurposing the CRISPR-...
  8. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...MG1655 and its derivatives DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more ... two individual isolates, the K-12 strain and the B strain. K-12 was isolated from a patient in 1920 and...likely isolated in 1918 but was first referred to as “B strain” in 1942. The BL21 strain and derivatives are... are the most common examples of the E. coli B strain. Common E. coli strains used in the lab Most of...HB101 Streptomycin Hybrid of E. coli K12 and E. coli B (mostly K12, though), common lab strain for cloning...cloning and storage strain. F- Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ- e14- mcrA0 relA1 ...sequences with the potential to recombine.  F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697...
  9. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...expression in hematopoietic stem cells, T cells, and B cells is low. Infection of these cells with VSV-G ...//doi.org/10.1093/hmg/10.19.2109 Sandrin V, Boson B, Salmon P, Gay W, Nègre D, Le Grand R, Trono D, Cosset...in cell lines expressing galactosyl(alpha1-3)galactosyl (alphaGal) sugars were less stable than viruses...express these sugars. (Takeuchi et al., 1997). The alphaGal sugars end up in the envelope and are targets ...targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated ..., and spumaviruses to human serum by galactosyl(alpha1-3)galactosylation. Journal of virology 71:6174–...
  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...a polypeptide chain, except if their names have a B, J, X, or Z. Some alternative, similar apps: Findings...restriction site to a primer, you only need to skim the alphabet on the side of the screen to find the sequence...
  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar Compartment...then be tracked along the secretory pathway (Figure B). The RUSH system has already been used to study transport...
Showing: 1 - 14 of 14 results