...10.1016/j.neuron.2016.01.040 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled...consist of a trimer of three subunits: alpha (Gα), beta (Gβ), and gamma (Gγ). G proteins are divided into...et al., 2015). PRESTO-TANGO uses a modified Tango beta-arrestin recruitment assay to measure receptor activation... In this assay, binding of an agonist recruits a beta-arrestin-TEV protease fusion to a receptor-transcription...kit consists of 20 plasmids encoding 14 alpha, 2 beta, and 4 gamma subunits of the heterotrimeric G protein...

...10.1038/s41589-020-0535-8 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled...complex consists of a trimer of three subunits: alpha, beta, and gamma (Gα, Gβ, and Gγ), collectively known ...or BRET2. With binding of the drug, the alpha and beta subunits dissociate resulting in decreased BRET2... 950 different human genes encoding GPCRs (Takeda S et al., 2002) and many downstream complexes involved...

... population. Yosef et al.’s proof-of-concept work targeted two distinct beta-lactam resistance genes, ...certain bacteria (Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance genes in E. coli...CRISPR antimicrobials successfully resensitized a beta-lactam resistant population to the antibiotic, despite... et al. used a phagemid system to target virulent S. aureus, a common hospital-borne infection. Two rounds...phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just 24 hours.   ...antibiotic-resistant bacteria.” Proc Natl Acad Sci U S A. 9;112(23):7267-72. (2015). PubMed: PMID 26060300...

...whose entire complementary strand was degraded. Beta: Beta protects the ssDNA created by Exo and promotes...system has three components (Figure 1): 1) Exo, 2) Beta, and 3) Gam. All three are required for recombineering...recombineering with a dsDNA substrate; however, only Beta is required when generating a modification with an... a complementary ssDNA target in the cell.  Only Beta expression is required for recombineering with an...).  In some of these strains, expression of exo, beta and gam is tightly regulated by the endogenous phage...to re-nature and again repress expression of exo, beta and gam.  A major advantage of using this method...-100 nucleotides long with the desired alteration(s) located in the center of the sequence. Since lambda...

...ubiquitous. human beta actin General expression mRNA Mammalian promoter from beta actin gene Constitutive...  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor. TRE General...interest will be expressed.      The RNA polymerase(s) RNA is transcribed from DNA using an RNA polymerase...

...interactions at a promoter in the well-characterized beta globin locus. The two methods had similar rates ...interactions at five sites in the well-characterized beta-globin locus were analyzed with CAPTURE 2.0, with...biotin acceptor site BirA, a biotin ligase a gRNA(s) targeting a single genomic location of interest  ... Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells Table 1: ...methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate of detecting chromatin interactions...they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing and high resolution allowed the Xu Lab...Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In ...

... of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss. Nature, 2010. 464(7292): ...reprogramming of adult pancreatic exocrine cells to beta-cells. Nature, 2008. 455(7213): p. 627-32. PubMed...861-72. PubMed PMID: 18035408. 3. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from...temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234-9. PubMed PMID: 21821793... of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848-57. PubMed PMID: 22645348...10): p. 1836-46. PubMed PMID: 22976355. 34. Wang, S., et al., Differentiation of human induced pluripotent...p. 82-94. PubMed PMID: 23329641. 36. Jopling, C., S. Boue, and J.C. Izpisua Belmonte, Dedifferentiation...

...Naldini et al. transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression...common hematopoietic disorders sickle cell anemia and beta-thalassemia. Lentiviral vectors are promising agents... Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros...Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467(7313):318-22....

...consisting of a CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains...//doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

...often have the topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to ... colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia virus DNA topoisomerase...coli is sequence specific."  Proc Natl Acad Sci U S A.  1991 Nov 15;88(22):10104-8. PubMed PMID: 1658796...synthesis using vaccinia DNA topoisomerase. Shuman S. J Biol Chem.  1994 Dec 23;269(51):32678-84. PubMed...

...References Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, ...such as the P.1 (Gamma variant) and the B.1.351 (Beta variant) were much less effectively neutralized....

...protein labeling and signal amplification. Zhou S, Feng S, Brown D, Huang B. PLoS One. 2020 Bo Huang Yellow...editing (Feng Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et al. Improved split fluorescent...according to the number of strands in the canonical beta-barrel structure. Most frequently, the proteins ...oligomerisation. Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP. FEBS Lett. 2017 Dan Mulvihill mVenus...visualization of endogenous proteins and synapses. Feng S et al. Commun Biol. 2019 Bo Huang Near-Infrared ...protein-protein interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol....

...Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G....High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label norepinephrine...and resources References Dayton, R. D., Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer...doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C. M., Leib... Leib, R. D., Cirolia, G., Thomas, D., Stamnes, S., Holt, K., Sinn, P., May, A. P., & Paulk, N. K. (2020...

...efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM, Collins...by secondary structure. It is thought that poly(A)s are used to control the cellular concentration of ...

...Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J. Beta-galactosidase gene fusions for analyzing gene expression... heterologous systems (e.g. mammalian proteins in S. cerevisiae). While this may be difficult to resolve...1985; 43:729-736. PubMed PMID: 3907859. 4. Fields, S., Song, O. A novel genetic system to detect protein-protein...membrane protein interactions. Proc Natl Acad Sci U S A. 2004; 101(33):12242–12247. Pubmed PMID: 15299147...

...and happy optimizing!  Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov...Resources References Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis of Transduction Efficiency...10.1089/hum.2009.169 Dudek, A. M., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette... https://doi.org/10.1038/s41434-019-0075-6 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov...number of different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression...10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa, Y.,...Fong, D. M., Mouravlev, A., Young, D., & O’Carroll, S. J. (2019). Astrocyte-selective AAV gene therapy through...

...in post-amputation planarians after knockdown of beta-catenin. Other gene targets do not require amputation... http://abt.ucpress.edu/content/79/3/208 2. Fire, S. Xu, M.K. Montgomery, S.A. Kostas, S.E. Driver, C....

...human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature communications 7 (2016). PubMed.... 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed...

...don’t always translate to eukaryotes. For example, beta-lactam antibiotics like penicillin and ampicillin... can be used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is ...

...orthogonal nuclease-inactive Cas9's (dCas9) from S. pyogenes, N. meningitidis and S. thermophilus which have been...Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules...favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112...620nm) portions of the spectrum. PhD student Benjamin S. Padman recently developed a mitochondrially-targeted...context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for...