Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
Type
Blog Post
Published
July 8, 2025, 1:15 p.m.
...generated using type IIS restriction enzymes (BsaI, BbsI/BpiI, BsmBI), which recognize asymmetric DNA sequences...each genetic part. (A) Example Level 0 assembly. BpiI recognition sites and four-base overhangs flank ...