Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 7 results for: Cascade-Cas3

Showing: 1 - 7 of 7 results

  1. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...unique capabilities of Cas3 and its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's ...compositions of the Cascade complex. Created with BioRender.com.   The Cascade complex Cascade is an important...recruitment by Cascade, Cas3 nicks the non-target strand. The helicase domain is then activated, and Cas3 begins...pulled through the Cas3. This mechanism is important for Cas3’s biggest trick. Cas3 likes to bluff, making... is waiting for its chance in the spotlight — Cas3. Cas3 can induce genomic alterations on a scale that...multi-Cas complex called Cascade (CRISPR-associated complex for antiviral defense). Cascade is brought to the...important component of the CRISPR Cas3 system. Most Cascade complexes are comprised of a combination of Cas5...

  2. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...attributed to the multi-Cas effector, Cascade. Developing tools using a Cascade complex is tricky, as you need...sections of DNA thanks to the dual action power of Cas3. Cas3 functions as both a helicase and a nuclease, ...components present in the Cascade complex. Figure 2: Schematic of a Cascade complex (blue subunits...systems use multi-Cas effectors, referred to as Cascade (CRISPR-associated complex for antiviral defense...use single protein Cas effectors, like Cas9. The Cascade complex doesn’t usually carry out the function ...unwinding and cutting up DNA after recruitment by Cascade. As you might imagine, this is handy for creating... employed as CRISPR transposases, by leaving out Cas3. Type I is further subdivided into subtypes A–G....

  3. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ... CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type V-K CAST systems...systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K systems; Cascade shown) combines with...Using CRISPR-Transposons Degrading DNA with Cascade-Cas3 Typing CRISPR Systems Prime Editing: Adding ...

  4. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...large genome edits using transposases or the Cascade-Cas3 system. We also describe newly engineered Cas9s...

  5. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...systems, however, Cascade from VchINT does not recruit other nucleases (such as Cas3) or have its own ...requirement for multiple Cas proteins to form the Cascade targeting complex is a hallmark of Type I CRISPR-Cas...proteins (TniQ, Cas8, Cas7, Cas6) that forms the QCascade DNA-targeting module together with the crRNA.  ... upstream 2-bp PAM sequence for recognition by QCascade; although 5’-CC-3’ is one of the best PAMs for...recognized by the system. Guided by the crRNA, QCascade complexes survey the genome and bind target DNA...downstream of the genomic target site bound by QCascade (Fig. 1B). The process also creates a 5-bp target-site...

  7. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...from gut. While still far from the clinic, CRISPR-Cas3 systems have been used successfully in mice for ...Clostridioides difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials. mBio 11: . https://doi.org/10.1128...1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting module with the crRNA, (3) three...
Showing: 1 - 7 of 7 results