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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3 results for: Cascade-Cas3

Showing: 1 - 3 of 3 results

  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3 Browse CRISPR...Blog Posts Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates ...created using Cas3 have fewer off-target alterations and require only a single gRNA. Cas3 must be paired..., Cas8, and Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. After nicking the...components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an...an all-in-one approach and include Cas3 and Cascade on one single plasmid, though many still separate ...individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse...

  2. CRISPR Guide

    Type
    Collection
    ...enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is ...Figure 14: Overview of editing with Cas3 Browse Plasmids: Cascade-Cas3 Want more information on the wide...separate Cas enzymes that form a complex called Cascade (see Cas3 for more details), while type V-K systems ... Degrading DNA with Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand...deletions generated by Cas9 systems. Cas3 must be paired with the Cascade ( C RISPR- as sociated c omplex ...recruited to target DNA using a gRNA and recruits Cas3. Cas3 nicks the non-targeting strand and starts degrading... strand is cleaved non-specifically by Cas3 along the way. Cas3 initiates a final DSB, either due to a...

  3. CRISPR Plasmids and Resources

    Type
    Collection
    ... without promoting DSB formation. Delete NEW Cascade-Cas3 generates large genomic deletions. Insert NEW...
Showing: 1 - 3 of 3 results