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We narrowed to 37 results for: Cascade-Cas3

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Showing: 1 - 20 of 37 results

  1. pCRISPR-Cas3

    Plasmid
    #209427
    Purpose
    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes
    Depositor
    Tilmann Weber
    Publication
    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    Use
    CRISPR and Synthetic Biology
    Promoter
    PtipA; crRNA under control of PermE*
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209427-plasmid-map-sequence-id-416703

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  2. pJW31

    Plasmid
    #136423
    Purpose
    Contains genes of Type I-C CRISPR-Cas system for inducible expression and chromosomal integration at attTn7
    Depositor
    Joseph Bondy-Denomy
    Publication
    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas5
    Cas8
    Cas7
    Cas3
    Cas3
    Use
    CRISPR
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136423-plasmid-map-sequence-id-270560

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  5. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

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  6. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid
    #134923
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134923-plasmid-map-sequence-id-267593

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  7. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid
    #204619
    Purpose
    Expression vector of Cascade and Cas3. Puromycin selectable.
    Depositor
    Akitsu Hotta
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    Use
    CRISPR
    Tags
    Each protein has C- and N-terminal SV40 NLS
    Promoter
    EF1a
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    204619-plasmid-map-sequence-id-404756

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  8. Cas3-Cascade-all-in-one-BSD

    Plasmid
    #260190
    Purpose
    All in one Cas3 and Cascade expression plasmid with blasticidin selection marker
    Depositor
    Leopold Parts
    Insert
    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    260190-plasmid-map-sequence-id-520981

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  9. Inducible-Cas3-Cascade-all-in-one-BSD

    Plasmid
    #260191
    Purpose
    Doxycyline inducible all-in-one Cas3 and Cascade expression plasmid with blasticidin selection marker
    Depositor
    Leopold Parts
    Insert
    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    Sept. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    260191-plasmid-map-sequence-id-521643

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  12. pCAG-All-in-one-hCascade

    Plasmid
    #134919
    Purpose
    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    134919-plasmid-map-sequence-id-272938

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  14. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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  15. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid
    #134924
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134924-plasmid-map-sequence-id-267600

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  18. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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Showing: 1 - 20 of 37 results