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Showing: 1 - 5 of 5 results
  1. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...protein (the CBP), and a partition NTPase (Schumacher, 2012).  During cell division, CBPs bind to the ...daughter cell. When plasmids are compatible, different CBPs bind to each plasmid type, and different NTPases...centromere-like binding region compete for the same CBPs and NTPase to correctly partition plasmids to each...each daughter cell. When there are not enough CBPs and NTPase to go around, the plasmids are randomly ...incompatibility is due to an identification issue: the CBPs in the cell can’t tell the difference between plasmids...
  2. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... tags Tag Epitope Mass (kDa) Function Notes CBP KRRWKKNFIAVSAANRFKKISSSGAL 4 Affinity and Purification...combo tag comprised of a calmodulin binding peptide (CBP), a TEV cleavage site (more on that in a moment),...
  3. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...with other members of the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase...
  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...C-terminal tags (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing...
Showing: 1 - 5 of 5 results