Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 11 results for: Cd4

Showing: 1 - 11 of 11 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...out all CD8+ T cells from a mix of CD8+ T cells, CD4+ T cells, B cells, and dendritic cells. In this case...except CD8+ T cells. We might choose the markers CD4, CD19, and CD11c, which would label everything but...excluded, or “dumped”. From left to right: B cell, CD4+ T cell, dendritic cell, CD8+ T cell. Created using...

  2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...HIV-1 infects cells in the immune system, notably CD4+ T-cells, and eventually leads to acquired immunodeficiency...T-cells to see if they could be rescued by CRISPR. CD4+ T-cells were isolated from healthy individuals, ...reduced the HIV-1 copy number by over 50% in isolated CD4+ T-cells from two HIV-1 infected patients. Although...

  3. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...focus on characterizing those epitopes as CD8 or CD4 epitopes, as well as their potential binding affinity... or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can be further...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ... substitute for HIV-1 Env, which can only infect CD4+ cells. 3rd generation lentiviral packaging system...the first clinical trial with a lentiviral vector. CD4+ T-cells from patients with HIV-1 were transduced...

  6. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...markers that define broader cell populations (e.g., CD4, CD8b, CD11b, CD19), are less likely to require an...

  8. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...effector immune cells. TGFβ is also known to change CD4+ Th cells into regulatory cells to suppress function...metastasis. Evading Recognition Tumor cells over-express CD47 to escape recognition by circulating macrophages...

  9. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...protein for the HEK 293T cells to express: CD45. Upon expression, CD45 is incorporated into the plasma membrane... manage to express CD45. Thus, if transduction efficiency is much higher than CD45 expression, this could...our flow experiment, we'll want to identify CD45+ cells (CD45 expression) and assess our transduction efficiency...our protein of interest: CD45. I am using a conjugated antibody to detect CD45, and I'll need to choose...transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason for having... fluorophore my antibody is conjugated to. Since CD45 is a common marker, finding an antibody already ...transgenic protein. We don't know yet how abundantly CD45 will be expressed on the transduced cells, so it's...
Showing: 1 - 11 of 11 results