We narrowed to 11 results for: Cd4

...HIV-1 infects cells in the immune system, notably CD4+ T-cells, and eventually leads to acquired immunodeficiency...T-cells to see if they could be rescued by CRISPR. CD4+ T-cells were isolated from healthy individuals, ...reduced the HIV-1 copy number by over 50% in isolated CD4+ T-cells from two HIV-1 infected patients. Although...

...out all CD8+ T cells from a mix of CD8+ T cells, CD4+ T cells, B cells, and dendritic cells. In this case...except CD8+ T cells. We might choose the markers CD4, CD19, and CD11c, which would label everything but...excluded, or “dumped”. From left to right: B cell, CD4+ T cell, dendritic cell, CD8+ T cell. Created using...

...focus on characterizing those epitopes as CD8 or CD4 epitopes, as well as their potential binding affinity... or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can be further...

... substitute for HIV-1 Env, which can only infect CD4+ cells. 3rd generation lentiviral packaging system...the first clinical trial with a lentiviral vector. CD4+ T-cells from patients with HIV-1 were transduced...

...systems were benchmarked on positive control genes in CD4+ and CD8+ T cells. To demonstrate the power of these...

...markers that define broader cell populations (e.g., CD4, CD8b, CD11b, CD19), are less likely to require an...

... et al. "A dynamic map of antigen recognition by CD4 T cells at the site of Leishmania major infection...

...effector immune cells. TGFβ is also known to change CD4+ Th cells into regulatory cells to suppress function...metastasis. Evading Recognition Tumor cells over-express CD47 to escape recognition by circulating macrophages...

...protein for the HEK 293T cells to express: CD45. Upon expression, CD45 is incorporated into the plasma membrane... manage to express CD45. Thus, if transduction efficiency is much higher than CD45 expression, this could...our flow experiment, we'll want to identify CD45+ cells (CD45 expression) and assess our transduction efficiency...our protein of interest: CD45. I am using a conjugated antibody to detect CD45, and I'll need to choose...transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason for having... fluorophore my antibody is conjugated to. Since CD45 is a common marker, finding an antibody already ...transgenic protein. We don't know yet how abundantly CD45 will be expressed on the transduced cells, so it's...

...see our ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology. Funding agencies ...

...insertion of a fluorescent protein are the tagging of Ccd42 (Bendezú et al., 2015) and the alpha subunit of ...