Plasmids 101: The Promoter Region – Let's Go!
Type
Blog Post
Published
April 3, 2014, 8:05 p.m.
...hybrid mammalian promoter
Constitutive
Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin... trying to pick your perfect promoter!
Eukaryotic Promoters
Promoter
Primarily used for
RNA ...ubiquitous.
human beta actin
General expression
mRNA
Mammalian promoter from beta actin gene
Constitutive
...activate promoter.
Ac5
General expression
mRNA
Strong insect promoter from Drosophila Actin 5c gene
....
In practice, the term "promoter" describes the combination of the promoter (RNA polymerase binding site...recognize and bind to specific promoter elements. This means that the promoter present in your plasmid backbone...RNAP II promoter, whereas small RNAs (such as shRNA) are transcribed from the RNAP III promoters. This ...