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We narrowed to 10 results for: Cre-dependent ChR2 expression

Showing: 1 - 10 of 10 results

  1. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo...serotypes, as well as a new Cre dependent GFP control plasmid for expression in forebrain GABA-ergic interneurons...available in AAV1 for Flp-dependent expression of ChR2(H134R) bound to an mCherry reporter. We now also...GFP dependent Cre. The two segments come together in the presence of GFP to form a functional Cre enzyme...preps SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins ...2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV...collection to include two new ChR2 constructs that allow for targeted expression. Plasmid 75470 is now available...

  2. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex of a mouse. Upon connection... the length of the fiber optic for the implant depends on the dorsal/ventral location of your region of... light-gated cation channel, Channelrhodopsin 2 (ChR2), exists in several states: closed/light receptive.... If you deliver a continuous stream of photons, Chr2 will open but will not be given an opportunity to...future. Now that your animals are all wired up and expressing your opsin of interest, it’s time to get to the... with diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream... rate of movement of the animal significantly increases. Before you get your experiment started, you will...

  3. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...shown that some channels, such as ChR2 and NpHR, can even be expressed simultaneously and still be individually...membrane when exposed to light. Neuronal firing depends on these charged particles and whether they make...will fire a signal. The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement... like spontaneous swimming. To do so, the lab expressed the LiGluR opsin in different subsets of spinal...spinal cord neurons using the Gal4/UAS system. This created fish lines with different groups of neurons that...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... a Drd1-Cre transgenic mouse to deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified... injected Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a Cre-dependent IL-1 beta...beta overexpression cassette. Expression of Cre in these neurons turns on overexpression of IL-1 beta. They...Channelrhodopsin 2 (ChR2) reduced glutamate transmission from the mPFC projections to the NAc and decreased the locomotion... investigated the injection of AAV8 particles expressing an activating chemogentic receptor (hM3Dq) into...at al. 2014). Addgene has several recombinase-expressing ready-made AAV vectors which can be used in numerous...researchers found that injecting lentiviral vectors expressing BDNF in the hippocampus causes a shift towards...

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...opsins to operate at increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to...Invasiveness  Choosing the best set of tools therefore depends on what you are looking to study. Figure ...delivery is the same in both cases, but activation depends on small molecule activation (chemogenetics) or...optogenetic and chemogenetic tools can have their expression targeted to specific tissues, cell types or even...restricted to illuminated region Impacts all cells expressing receptors Invasiveness Mice require permanent...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...continual expression of both dCas9 and the gRNA. This expression can vary significantly depending on transgene...easier to clone, express, and purify compared to antibodies. Nanobodies are easily expressed in the cytosol...#140702) The pMAK461-64 plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction...sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted syn-FLEX-axon-jYCaMP1s...syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype PHP.V1 that exhibits...this AAV at Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, ... the mCitrine gene. mCherry is constitutively expressed. Cells are then cultured and FACS sorted. Image...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...with an appropriate mouse line expressing a particular cre, viral expression of your opsin is no longer required...steps in the coming weeks. Expression of the optogenetic constructs Expression of your desired optogenetic...promoter-directed expression alone is extremely limited. Therefore, highly expressing, generic neuronal...opsin allows cell type specificity dependent on the promoter of the cre recombinase while still taking advantage...Finally, expression of the opsin in a mouse line will likely be less than that of viral expression but may...stimulation. Testing opsin expression  Regardless of the method of expression of your opsin gene, it is...Viruses that only express the fluorescent proteins are used as a critical control. Depending on the AAV serotype...

  8. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...strong opsin expression in specific neuronal cells? This can be achieved using Cre-dependent viruses, such...cells with an inactive opsin gene; in Cre expressing cells, however, Cre induces the recombination of the ...channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing cells. The...combination with transgenic animals or rAAVs expressing Cre under a specific cell-type promoter. Once injected...not naturally light-sensitive, so the selective expression of opsin genes in targeted cells makes it possible...How to get an opsin into the brain In order to express opsins within the brain, researchers inject genetically...relatively stable extra-chromosomally long-term expression and low ability to induce innate immune responses...

  9. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...directs strong, ubiquitous expression. This is a Cre-dependent switch that expresses nuclear mCherry in the...the absence of Cre, and expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium sensors ...to release a Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre...ubiquitous expression. pAAV-mDlx-NLS-mRuby2 (99130-AAVrg): The Dlx element directs expression in forebrain...see below), in addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV inventory...pAAV-EF1a-fDIO-Cre (121675-AAV9, 121675-AAVrg)  pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1...entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors and ...

  10. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2...only interpret their results, but also confirm expression in target cells. While the various AAV serotypes... can be used to achieve different patterns of expression, researchers often need to determine these transduction...calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several new options are also...under control of the CAG promoter for ubiquitous expression. (For more details on this technology, see our...Fon hChR2(H134R)-EYFP 26975 AAVrg pAAV-CaMKIIa-hChR2(H134R)-mCherry 26976 AAV5 pAAV-hSyn-hChR2(H134R...packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)...
Showing: 1 - 10 of 10 results