...is also placed in the hood. Finally, a sterile 2X DMEM solution (diluted from 10X and containing both Hepes...3 ml of melted agarose are mixed with 3 ml of 2X DMEM in a 50 ml sterile tube (for 60 mm dishes only 1.5...onto the plate. We test the (capped) 1:1 agarose/DMEM solution against a part of the body very sensitive...provide a greater surface area (the amount of agarose/DMEM should be proportionately increased). For some cells...

...defective tinman gene fail to develop a heart (Bodmer, 1993). Bodmer named the gene after the Wizard of Oz character...formation of the heart amongst other things. Rolf Bodmer discovered Tinman when he observed that flies with...7.7b (1993): 1325-1340. PubMed PMID: 8101173.   Bodmer, Rolf. "The gene tinman is required for specification...

...other files relevant to the project. README: The README.txt document is invaluable. In the top directory...

...mediated transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated..., often show low efficiency, while biolistic bombardment, which can have high efficiency, requires specialized...

...and me. To that end, here are my personal 10 commandments for making it all work – most of the time. #1...

...friends @iprophage @robincagle5 pic.twitter.com/52KQDMeSjO — Brianna Weiss (@briannadoesbio) June 22, 2019...