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We narrowed to 6 results for: DME

Showing: 1 - 6 of 6 results

  1. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...is also placed in the hood. Finally, a sterile 2X DMEM solution (diluted from 10X and containing both Hepes...3 ml of melted agarose are mixed with 3 ml of 2X DMEM in a 50 ml sterile tube (for 60 mm dishes only 1.5...onto the plate. We test the (capped) 1:1 agarose/DMEM solution against a part of the body very sensitive...provide a greater surface area (the amount of agarose/DMEM should be proportionately increased). For some cells...

  2. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...defective tinman gene fail to develop a heart (Bodmer, 1993). Bodmer named the gene after the Wizard of Oz character...formation of the heart amongst other things. Rolf Bodmer discovered Tinman when he observed that flies with...7.7b (1993): 1325-1340. PubMed PMID: 8101173.   Bodmer, Rolf. "The gene tinman is required for specification...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...mediated transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated..., often show low efficiency, while biolistic bombardment, which can have high efficiency, requires specialized...
Showing: 1 - 6 of 6 results