...the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation lentiviral backbone...

...other promoters, including ubiquitous ones such as EF-1α and CAG. Figure 2: Dual lentivirus system...

... PubMed Central PMCID: PMC3565066. 7. Quax, Tessa EF, et al. "Codon bias as a means to fine-tune gene ...

...encode phosphoserine, the Sep-tRNA synthetase, and EF-Sep – an optimized elongation factor that accepts...

...useful for a wide variety of experiments. Opto-RhoGEFs manipulate cell morphology and adhesion But what...pathways. Rho guanine-nucleotide exchange factors (GEFs) activate their partner Rho GTPase, leading to behaviors...Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1, or p63RhoGEF (which activate the Rho...iLID system (Mahlandt et al., 2023). These Opto-RhoGEFs provide a reversible and non-invasive way to activate...be ready for the spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic of...4.0 license.   Mahlandt et al. used these Opto-RhoGEFs to study cell junction integrity in the vascular..., van Buul, J. D., & Goedhart, J. (2023). Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly...

...information if those sources affirm or challenge their beliefs. I asked participants to read short abstracts ... to the abstracts that affirmed their personal beliefs even though all three texts were otherwise identical...for a study with findings that challenged their beliefs, they were much more likely to rate that study ...science literacy and education have more polarized beliefs on controversial science topics. Proceedings of...

...direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors (iEPs), a self-renewing...differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs by retroviral expression...CellTagging to uncover two distinct trajectories of MEFs undergoing iEP conversion, but CellTag could be ...

...genes that apparently contribute to virulence (Tollefson et al., 1996) – cell death has been reported when...10.1182/BLOOD-2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, ...

...you simply type in identifier keywords (Cas name, RefSeq ID, etc.) and you will see a table of protein entry...

...activate all 23,430 known coding isoforms from the RefSeq database. The key to developing the new system,...

...PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule...

...media and blog posts, lesson plans, or information briefs for policymakers. The rapidly changing landscape...

...Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA (2018) CRISPR-Cas12a target binding...

...pull-down procedure for the identification of unknown GEFs for small GTPases." Small GTPases 7.2 (2016): 93...

... to speak with individuals who had very strong beliefs in traditional medicine, especially when I knew...

... 269-272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs N., Yon W. Ebright, and Richard H. Ebright. "Site-specific...