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We narrowed to 20 results for: ER;

Showing: 1 - 20 of 20 results

  1. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes to the cytoplasm? Or more...absolute position requirements. For example, a KDEL ER retrieval sequence only functions at the extreme ...potential to interfere with the function of resident ER chaperones and may impact cellular function or possibly...complex-localized fusions from properly exiting the ER and fluorescing (see Figure 2) [5].  When using FPs... pathway, a dark pool that is mislocalized to the ER can be detected with immunofluorescence. This population...through the secretory pathway, and retained in the ER. FPs have been undeniably powerful and successful...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... the ER lumen reaches the sub-millimolar range, much higher than in the cytosol. Thus, for the ER you ...measuring the events-of-interest. Sensors suitable for ER measurements include: CEPIA (additional versions ... to simultaneously measure calcium changes in the ER, cytosol and mitochondria), the Cameleon-based FRET...

  3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...fluorescent protein to the Endoplasmic Reticulum (ER). If homodimerization occurs (most likely in an antiparallel...used as positive and negative controls for proper ER structure. Based on these data, we conclude that ...tagging. On the other hand, mScarlet-I shows correct ER labeling, which is good evidence that mScarlet-I ...

  4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ... lab's SARS-CoV-2 sybodies! References Geertsma ER (2013) FX Cloning: A Versatile High-Throughput Cloning...Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation of synthetic nanobodies...Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER, Dawson RJ, Seeger MA (2018) Synthetic single domain...

  5. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...communication specialist (you'll know me by my Tweeting — er, posting!) and our favorite content strategist, Jo...

  6. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...S, Pelham HRB (1986) An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein...A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016...

  7. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ... multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB) to the C-terminus of...ligand Tamoxifen is added. Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate...

  10. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... Figure 2: Two-color super-resolution imaging of ER and mitochondria reveals dynamic organelle interactions...interactions. a) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules ...of secretory proteins, from their synthesis in the ER to their arrival in their target compartment (e.g...stably localized in the donor compartment (e.g. the ER) and a reporter protein of interest (Figure A). Franck...and interactions between the endoplasmic reticulum (ER), mitochondria, cytoskeleton, and endosomes. These...

  14. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. ...

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...to label proteins moving to and from the nucleus, ER, mitochondria, stress granules, and more. For added...

  19. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...results, so you only need to pick your favorite (...er, whichever one uses assumptions that match your data...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... previously unsuspected genes with known roles in ER-associated degradation (ERAD) that positively regulate...
Showing: 1 - 20 of 20 results