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We narrowed to 35 results for: FLP

Showing: 1 - 20 of 35 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient The first...fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression...Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels...levels of genetic control. What is FLP FRT and how does it work? FLP recombinase, which is named after ...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes...(LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained in the...Andrea et al. 2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): ...

  2. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ... we are excited to add Flp-expression AAV to our recombinase AAV inventory. Flp is another useful recombinase...populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase...detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional studies And more! ...Serotype Name 55637  AAVrg pAAV-EF1a-Flpo 87306  AAV1 AAV pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors...55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Chemogenetics Plasmid Serotype...

  3. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...with low cross-reactivity, as well as low toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) ...with flies heterozygous for tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring...shows that the yeast recombinases KD, B2, B3 and FLP can be used together in Drosophila as a toolkit for...

  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available in AAV1. Plasmid...expression. Plasmid 75470 is now available in AAV1 for Flp-dependent expression of ChR2(H134R) bound to an mCherry...pAAV-EF1a-C-CreintG Connie Cepko 75469 AAV1 pAAV-EF1a-Flp-DOG-NW Connie Cepko Viral vectors coming soon...targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-CoV-2 protein...

  5. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-directed... conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) ...being analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to...

  6. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...of the site-specific recombinases (SSRs) Cre and FLP. SSRs bind DNA at target sites to induce site specific... events: Cre recombinase binds loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by...and a pair of FRT sites and include both Cre and FLP recombinases.   Figure 1 (modified from Schnutgen...

  7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...transgenic models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent...sequences, lox (recognized by Cre) or frt (recognized by Flp), to either flip or excise a gene that is located...leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify the amount of transgene...expression can occur in cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and ...

  8. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9...our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several new options... Serotype Name 87306 AAVrg, AAV5 AAV pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources on the Addgene...

  9. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo (55634-AAV1) pAAV-EF1a-Flpo (55637-AAV1) Viral...collection. Of note, we are excited to release a Flp-dependent Cre AAV in AAV9 and AAVrg. See our new ...

  11. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...Specificity can be achieved using specialized promoters, Flp or Cre dependent gene switches, and serotype selection...cell-specific gene expression and viral infection. Cre or Flp dependent switches control gene expression via switches...

  12. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...including our first far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype...

  13. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    .... It’s an alternative to the conventional Cre or Flp recombinases used to excise markers. It has the advantage...

  15. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT ...-responsive CreERT, the coupling of Cre-lox with FLP-FRT recombination to generate additional recombination... placed under different promoters, Brainbow and Flpbow can be used to label distinct cell populations....

  18. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Calcium...
Showing: 1 - 20 of 35 results