We narrowed to 7 results for: GCG

1. Plasmid Cloning by PCR

   Type

   Blog Post

   Published

   March 29, 2016, 2:30 p.m.

   ...'-TGGCATATCTCGAAGTACTGA-3'), then adding NotI (GCGGCCGC) and then TAAGCA to improve restriction enzyme.... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

2. Components of CRISPR/Cas9

   Type

   Blog Post

   Published

   Feb. 2, 2016, 5 p.m.

   ...reduced NAG binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant 3' NGAG SpCas9 VQR ...

3. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ... different PAM sites (NGG, NGAN, NGNG, NGAG, and NGCG). Since then, the Osamu Nureki Lab described a rationally...

4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...SpCas9 variants with alternative PAMs like NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the...

5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...VRER variant D1135V, G1218R, R1335E and T1337R NGCG The D1135E variant is far more selective for...

6. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...KKH TadA-TadA* NNNRRT 8 SpCas9-VRER TadA-TadA* NGCG 8  Tips for designing base editing experiments...

7. 15 Hot Plasmids from 2017

   Type

   Blog Post

   Published

   Jan. 9, 2018, 3:02 p.m.

   ...activity than BE3  85173  pBK-VRER-BE3  VRER-Cas9 (NGCG)    85174  pBK-YE1-BE3  SpCas9 (NGG)  Editing ...