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Showing: 1 - 8 of 8 results
  1. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...dot plot, we have A-B- cells, A-B+ cells, and A+B+ cells, but no cells that are A+B-.      Figure...population of B cells and dendritic cells (DCs) in the lower middle right. DCs and B cells are HLA-DR+ and ...case of Figure 2, one population is B- and another two are B+. Our A-B+ cells are in the lower right corner...negative for marker A and positive for marker B, or A-B+ cells. If you look at the dot plot in Figure ... plot has two populations that are B+ and one population that is B-. Created with biorender.com.   ...license.    In the third plot, anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers are...the amount of signal read from Marker A and Marker B. Created with biorender.com.   The goal of flow...
  2. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...of Monoclonal Antibodies and the Potential Use of HLA Haplotypes to Predict Vulnerable Patients. Frontiers...10.3389/fimmu.2022.885672Piché, M.-S., Mounho-Zamora, B., LeSauteur, L., & Burns-Naas, L. A. (2015). Chapter...Gutierrez, A. H., Martin, W. D., Terry, F. E., Roberts, B. J., Rosenberg, A. S., & De Groot, A. S. (2022). In...
  3. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...removing tubulin dimers from the microtubule body. B) Schematic of Opto-katanin’s use in cells. Images ... A) Schematic of Opto-RhoGEF design and activity. B) OptoTIAM (magenta) expressed in a monolayer of endothelial...be monitored by phosphatidic acid probe GFP-PASS. B) Time series of HEK 293T cells expressing plasma membrane-targeted..., Ochs, C. C., Jaber, Z., Gardner, K. H., & Chow, B. Y. (2022). Designing Single-Component Optogenetic.... Images adapted from Mahlandt et al., 2023, under CC-BY 4.0 license.   Mahlandt et al. used these Opto-RhoGEFs...and RhoA, respectively) using the iLID system (Mahlandt et al., 2023). These Opto-RhoGEFs provide a reversible...521. https://doi.org/10.1021/acssynbio.1c00604.  Mahlandt, E. K., Palacios Martínez, S., Arts, J. J., Tol...
  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...light, oxygen, and voltage (LOV) domains, phytochrome B (PhyB), and cryptochrome 2 (CRY2) (Zhang & Cui, 2015..., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan....org/10.1038/s41467-021-24759-5 Zhang, K., & Cui, B. (2015). Optogenetic control of intracellular signaling...light-activated cation channel, from the archaebacteria Chlamydomonas reinhardtii. When exposed to blue light, this...
  5. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...Schmidt G, Öztürk MA, Ernst L, Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light...L-arabinose drive. Dedicated to Edo, Armin and @KhammashLab. I hope you all enjoy it!Disclaimer: I had to...References and Resources References Romano E, Baumschlager A, Akmeriç EB, Palanisamy N, Houmani M, Schmidt...
  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ..., Collins JR, Cooper DN, Bohr VA, Vasquez KM. Non-B DNA-forming sequences and WRN deficiency independently...: 32338220. Crozat E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution of Multimeric Forms of Circular...978-1-60761-340-4_2. PMID: 19763938. Maucksch C, Bohla A, Hoffmann F, Schleef M, Aneja MK, Elfinger M, ...
  7. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger B, Hensel Z (2019) On the value of preprints: An early...preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (2022) A guide to preprinting...
  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...then be tracked along the secretory pathway (Figure B). The RUSH system has already been used to study transport...luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy...developed by the Johnson Lab . Characteristics of the pHlash reporter protein include: H+ specific response...fluorophore, a circularly permuted Venus. In the case of pHlash, the signal from the fluorophore is pH-dependent...
Showing: 1 - 8 of 8 results