Antibodies 101: Reading a Flow Plot
Type
Blog Post
Published
Feb. 6, 2024, 3:13 p.m.
...dot plot, we have A-B- cells, A-B+ cells, and A+B+ cells, but no cells that are A+B-.
Figure...population of B cells and dendritic cells (DCs) in the lower middle right. DCs and B cells are HLA-DR+ and ...case of Figure 2, one population is B- and another two are B+. Our A-B+ cells are in the lower right corner...negative for marker A and positive for marker B, or A-B+ cells. If you look at the dot plot in Figure ... plot has two populations that are B+ and one population that is B-. Created with biorender.com.
...license.
In the third plot, anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers are...the amount of signal read from Marker A and Marker B. Created with biorender.com.
The goal of flow...