....   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene confers resistance...decorative" title="Check icon" %} Satellite colonies Kanamycin Excellent No Bacteria and eukaryotes (G418) {%...

...precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi, and Eric A. ... PMID: 28356322 PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess ...

...previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee et al.(1...3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX).  Each PCR product from this ...

...instead use G418. This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin...

...serious-  competition with our corn toss boards, Kan Jam, and Ladder Ball (I finally discovered my hidden...

...PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test for sickle-cell anemia...

...frequencies in the central nervous system (CNS) (Wu, Asokan, et al., 2006). AAV1 has also been found to transduce...kidney, and retinal cells (Issa et al., 2023; Wu, Asokan, et al., 2006). AAV2 capsids are available as plasmids...kidney, and retinal cells (Issa et al., 2023; Wu, Asokan, et al., 2006). AAV8 capsids are available as plasmids...varies by species and route of administration (Wu, Asokan, et al., 2006). Want to know if a capsid is a good...doi.org/10.2337/diabetes.55.04.06.db05-0927 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated...

...with the cells’ endogenous ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well...Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method...essential proteins (Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for ...create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor...

...serotype can affect spread from the injection site  (Kanaan et al., 2017), axonal transport  (Murlidharan et...cells12050785 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-associated viral vectors...5), 1–41. https://doi.org/10.3390/cells12050785 Kanaan, N. M., Sellnow, R. C., Boye, S. L., Coberly, B...pharmaceutics13050750 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-associated viral vectors...

...signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower DOL tend...intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness...994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., Nozaki,...

...backbone contains a kanamycin resistance cassette, you would plate the cells on kanamycin-containing agar..... Only cells carrying plasmids that contain a kanamycin resistance cassette will grow. However, often ...

...activity after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy...Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular...two-photon laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in...

...Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license. Figure 2 gives a...1406488. PubMed Central PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of ...PubMed Central PMCID: PMC4515362 Robinson, W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the...

... based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current...  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag 182 Benzylcytosine...

...were first expressed in E. coli in the 1980s  (Kanabrocki et al 1986). Much later, it was shown that putting...her on Twitter @jessicapolka.     References 1. Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization...

... with Dr. Stephani Page, the founder of the #BLACKandSTEM Twitter community. Dr. Page shares her story...@ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM what do you do? — Stephani Page, PhD 👩🏾‍🔬...

...PMID: 30272679. 8. Hua, Kai, Xiaoping Tao, and Jian‐Kang Zhu. "Expanding the base editing scope in rice by...11.4 (2018): 627-630. PubMed PMID: 29476916. 10. Kang, Beum-Chang, et al. "Precision genome engineering...

...contrast than previously developed NIR split probes (Tchekanda et al., 2014, Filonov et al., 2013). FRET biosensors...fluorescent nanobodies. Nat Methods submitted (2021). Tchekanda, E., Sivanesan, D. & Michnick, S.W. An infrared...

...acid to Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also shown that the Q system is also.... Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling gene expression with the Q repressible...

... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow...