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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 19 results for: MS2;

Showing: 1 - 19 of 19 results

  1. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ... sgRNA containing alternating MS2 and PP7 sequences (sgRNA-Sirius-4X(MS2-PP7)) that result in yellow fluorescence...) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius-8XPP7, and sgRNA-Sirius-4X(MS2-PP7) showing recruitment ...scaffolds that contain two hairpin sequences (either MS2, PP7, or boxB) that each recruits a different fluorescent...unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences inserted into the sgRNA generates...green, or red colors for labeling. A combination of MS2, PP7, or boxB sequences in the same sgRNA will generate...’ end of the gRNA. Because of the success of the MS2 and PP7 RNA hairpin sequences in CRISPRainbow, they...for further development. They inserted an octet of MS2 hairpins into the gRNA tetraloop and went through...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...Blue MS2-MS2 Green PP7-PP7 Red boxB-boxB Cyan MS2-PP7 Yellow PP7-boxB Magenta boxB-MS2 White...White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently labeled Cas9’s come with...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...achieved by using multiple copies of the PP7 and MS2 coat protein binding sites to tether many copies ...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains... coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein binding sites to tether... study to target various RNA molecules - such as MS2 stem loops, PPL stem loops, the Spinach2 aptamer,...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...to recruit an sgRNA with MS2 hairpin binding sites, which then recruit an MS2-fused hyperactive variant...

  15. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...Int J Mol Sci., 21(18): 6461 (2020). 10.3390/ijms21186461 Lin, S., Staahl, B. T., Alla, R. K., Doudna,...homology-dependent repair. Nat. Com., 9-1133 (2018). 10.3390/ijms21186461 Additional Resources on the Addgene Blog Check...

  16. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...Int J Mol Sci 24:5994. https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung S, et al (2021) Nanobodies ...

  17. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...Mol Sci. 2023 Jan 13;24(2):1563. doi: 10.3390/ijms24021563 Resources on Addgene.org CRISPR Plasmids and...

  19. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084 Additional resources on the Addgene blog Learn...
Showing: 1 - 19 of 19 results