...cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote... chance. Here was an opportunity to see Shinya Yamanaka celebrate the tenth anniversary of his landmark... and cMyc (the so called OSKM factors). As the Yamanaka lab's primary Addgene contact for many years, ...community – a community largely created due to Yamanaka’s seminal work. It didn't take very long to realize...realize that the effect of Dr. Yamanaka's work has been profound, and this is a rare instance in which...thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz Studer...stepped back to give a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with ...

...generated from mouse fibroblasts in 2006 in Shinya Yamanaka’s lab, where the reprogramming factors were delivered... was taken forward the next year when both the Yamanaka and Thomson labs showed the successful generation...These stellar works led to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs...PubMed PMID: 17989069. 8. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from mouse...

...General Hospital. A little over a decade back when Yamanaka and colleagues reported that it is possible to... (iPSCs) by the addition of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative...19668191. PubMed Central PMCID: PMC2917235. 11. Nakagawa, M., et al., Promotion of direct reprogramming... Enhanced Reprogramming Find plasmids from the Yamanaka Lab ...

...signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower DOL tend...intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness...994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., Nozaki,...

...couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used...(basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively, if you prefer Golden...25902487. PubMed Central PMCID: PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors...

...short dsRNA. However, it is important to note that naked and unmodified dsRNA elicits immunogenic reactions...Physcomitrella) patens (Bezanilla et al., 2005; Nakaoka et al., 2012).     A new positive selection-based... 34:634–636 . https://doi.org/10.1038/nbt.3567 Nakaoka Y, Miki T, Fujioka R, Uehara R, Tomioka A, Obuse...

..., Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV,...Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F,...

...codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What is codon optimization? Codon...  https://doi.org/10.1016/j.molmed.2014.09.003 Nakamura Y (2000) Codon usage tabulated from international...

...intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels of the second messenger...23488798. PubMed Central PMCID: PMC3775879. Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin...

...for a longer time. The aerosols are invisible to naked eyes and hence cannot be detected until it leads...mycoplasma contamination Detection of mycoplasma by naked eyes or optical microscope can be very difficult...

...PubMed PMID: 18035408. 3. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from mouse...Vectors for Your Research Find Plasmids from the Yamanaka Lab ...

...Masayuki, Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression...Find reprogramming plasmids here Check out the Yamanaka paper that started the field of iPSCs Find gene-specific...

..., H., Yuasa, S., Takeda, S., Hasegawa, I., and Nakahara, K. (2011). Local and retrograde gene transfer...Central PMCID: PMC5102064. Roberts, T. F., Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah...

...dye front (and many ladders!) are visible to the naked eye.   If you see a smiley face in your gel, or ...you can use a colorimetric ladder, visible to the naked eye; a fluorescent ladder compatible with your imaging...

...brief yet bright burst of light, visible to the naked eye, at regular intervals. This innovation system...

... pluripotent stem cells. Plasmids from Shinya Yamanaka's lab and James Thomson's lab were the most frequently...

...transposons combine the advantages of viruses and naked DNA while eliminating some of the drawbacks. Specifically...

...References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. 1987 Dec; 169 (12): 5429–33. ...

...from cryptic promoters in the plasmid backbone (Giannaks et al 2003, Bert et al 2000). In 2000, Peter Cockerill...Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et al. BioTechniques. 2003. PubMed PMID: 12951775...

...pigment reporter whose expresion is visible to the naked eye in plants. When expressed, the construct produces...