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We narrowed to 34 results for: NAK

Showing: 1 - 20 of 34 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote... chance. Here was an opportunity to see Shinya Yamanaka celebrate the tenth anniversary of his landmark... and cMyc (the so called OSKM factors). As the Yamanaka lab's primary Addgene contact for many years, ...community – a community largely created due to Yamanaka’s seminal work. It didn't take very long to realize...realize that the effect of Dr. Yamanaka's work has been profound, and this is a rare instance in which...thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz Studer...stepped back to give a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with ...

  2. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...generated from mouse fibroblasts in 2006 in Shinya Yamanaka’s lab, where the reprogramming factors were delivered... was taken forward the next year when both the Yamanaka and Thomson labs showed the successful generation...These stellar works led to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs...PubMed PMID: 17989069. 8. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from mouse...

  3. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...General Hospital. A little over a decade back when Yamanaka and colleagues reported that it is possible to... (iPSCs) by the addition of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative...19668191. PubMed Central PMCID: PMC2917235. 11. Nakagawa, M., et al., Promotion of direct reprogramming... Enhanced Reprogramming Find plasmids from the Yamanaka Lab ...

  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower DOL tend...intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness...994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., Nozaki,...

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used...(basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively, if you prefer Golden...25902487. PubMed Central PMCID: PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...short dsRNA. However, it is important to note that naked and unmodified dsRNA elicits immunogenic reactions...Physcomitrella) patens (Bezanilla et al., 2005; Nakaoka et al., 2012).     A new positive selection-based... 34:634–636 . https://doi.org/10.1038/nbt.3567 Nakaoka Y, Miki T, Fujioka R, Uehara R, Tomioka A, Obuse...

  7. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What is codon optimization? Codon...  https://doi.org/10.1016/j.molmed.2014.09.003 Nakamura Y (2000) Codon usage tabulated from international...

  8. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ..., Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV,...Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F,...

  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...intracellular viral DNA dynamics in neuronal cells (Nilaratanakul et al., 2017) and levels of the second messenger...23488798. PubMed Central PMCID: PMC3775879. Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...Masayuki, Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression...Find reprogramming plasmids here Check out the Yamanaka paper that started the field of iPSCs Find gene-specific...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ..., H., Yuasa, S., Takeda, S., Hasegawa, I., and Nakahara, K. (2011). Local and retrograde gene transfer...Central PMCID: PMC5102064. Roberts, T. F., Hisey, E., Tanaka, M., Kearney, M., Chattree, G., Yang, C. F., Shah...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...dye front (and many ladders!) are visible to the naked eye.   If you see a smiley face in your gel, or ...you can use a colorimetric ladder, visible to the naked eye; a fluorescent ladder compatible with your imaging...

  15. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...brief yet bright burst of light, visible to the naked eye, at regular intervals. This innovation system...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...from cryptic promoters in the plasmid backbone (Giannaks et al 2003, Bert et al 2000). In 2000, Peter Cockerill...Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et al. BioTechniques. 2003. PubMed PMID: 12951775...
Showing: 1 - 20 of 34 results