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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 21 results for: PANC

Showing: 1 - 20 of 21 results

  1. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...represents the major difference between PACE and PANCE: for PANCE the phage progeny is not diluted out but gets...PACE) and Phage-Assisted Non-Continuous Evolution (PANCE) selection systems to engineer better and faster...selection, and replications per day.  The PACE and PANCE systems use M13 bacteriophage, which rely on the...and flexible adenine base editor Lower-stringency PANCE, followed by higher stringency PACE, resulted in...

  2. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...transdifferentiated in vivo to pancreatic β-like-cells with the expression of pancreatic and duodenal homoeobox... [13]:  Germ cells [14] Hepatocytes [15] Pancreatic β-cells [16] Intestinal tissue [17] Lung epithelial...rare in mammals, an example is observed in the pancreas when excess β-cell damage results in the transdifferentiation...cell types such as fibroblasts, hepatocytes, and pancreatic exocrine cells have been successfully transdifferentiated...differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells. Cell Res, 2009. 19(4.... 38. Thorel, F., et al., Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-...PubMed PMID: 3690668. 40. Ferber, S., et al., Pancreatic and duodenal homeobox gene 1 induces expression...

  3. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...process ensures that any potential sequence discrepancies can be caught prior to distribution. Catching...issue? At Addgene, a QC issue is defined as a discrepancy that we believe may affect the function of the...decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited to Addgene...resolve. Sequencing and assembly issues Sequence discrepancies are not always a result of mutations in the ...will contact the depositing lab to review the discrepancies. Often, the identified issues will not affect...requesting scientists aware of the identified discrepancy in the plasmid, and that it is not known to impact...plasmids and provide transparency in sequence discrepancies. While Addgene’s QC process adds time to the...

  4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...been shown to transduce skeletal muscle, cardiac, pancreas, smooth muscle, CNS, kidney, and retinal cells...been shown to transduce liver, skeletal muscle, pancreatic, and retinal cells (Issa et al., 2023). AAV9 ...pUCmini-iCAP-AAV9.452sub.LUNG1 Goertsen et al., 2022 Pancreas AAV6, AAV8 Wang et al., 2006 Retina 7M8 Dalkara... J., & Xiao, X. (2006). Widespread and Stable Pancreatic Gene Transfer by Adeno-Associated Virus Vectors...

  5. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...differentiation of β-cells in the the developing zebrafish pancreas. They first tagged these cells using plasmids....β-cells that originate in different regions of the pancreas and produce different levels of insulin (Hesselson...Distinct populations of quiescent and proliferative pancreatic β-cells identified by HOTcre mediated labeling...

  6. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...al.) Monitor gene expression in the developing pancreas by placing FP timer DsRed-E5 under the control...control of Neurog3 (a gene controlling pancreatic differentiation) in mouse embryos (Miyatsuka et al.) Distinguish...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    .... At Addgene, we use blastn to identify any discrepancies in Sanger sequences, such as mismatches, deletions...determine the potential effect of any nucleotide discrepancies. The blastp and blastx programs are optimized...

  8. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... used Mango to study RNA-protein complexes (Panchapakesan et al., 2017) and short non-coding RNAs (Autour...27446908. PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein...

  9. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...detection and diagnosis of different types of pancreatic cancer and highlighting which treatment would... mutations. This is a particular challenge in pancreatic cancer where one drug does not work for all patients...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...polycistronic cassettes and discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal... did not lead to the observed reprogramming discrepancies. The polycistronic piggyBac transposon vectors...

  12. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...
Showing: 1 - 20 of 21 results