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We narrowed to 4 results for: PLKO.1 puro cloning vector

Showing: 1 - 4 of 4 results
  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes...plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol...pSpCas9(BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses.... Find lentiGuide-Puro. pSpCas9n(BB)-2A-GFP (PX461) - Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n...2A-GFP (PX461). pSpCas9(BB)-2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your...
  2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're... Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested ...you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. In 2007 we continued...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two...negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral...
  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408...Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene of interest pLenti-puro (Plasmid...expression vectors (empty backbones) here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...interest with GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference...research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral...
  4. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ... to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our...cases bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope...fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes... multiple cloning site to insert YGOI, a bacterial selection cassette for growth and cloning in bacteria...will need accessory packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that ...
Showing: 1 - 4 of 4 results