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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 5 results for: PP7

Showing: 1 - 5 of 5 results

  1. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ... containing alternating MS2 and PP7 sequences (sgRNA-Sirius-4X(MS2-PP7)) that result in yellow fluorescence...created another sgRNA containing the PP7 hairpin sequence (sgRNA-Sirius-8XPP7) to recruit GFP to the loci of... sgRNA-Sirius-8XMS2, sgRNA-Sirius-8XPP7, and sgRNA-Sirius-4X(MS2-PP7) showing recruitment of different... that contain two hairpin sequences (either MS2, PP7, or boxB) that each recruits a different fluorescent... Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences inserted into the sgRNA generates...or red colors for labeling. A combination of MS2, PP7, or boxB sequences in the same sgRNA will generate... the gRNA. Because of the success of the MS2 and PP7 RNA hairpin sequences in CRISPRainbow, they knew ...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...Hairpin Blue MS2-MS2 Green PP7-PP7 Red boxB-boxB Cyan MS2-PP7 Yellow PP7-boxB Magenta boxB-MS2 ... White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently labeled Cas9’s come...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...binds to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP protein that binds...system are achieved by using multiple copies of the PP7 and MS2 coat protein binding sites to tether many...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...binds to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds...great specificity and resolution by using 6-12 phage PP7 coat protein binding sites and 24 copies of the MS2...
Showing: 1 - 5 of 5 results