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...RNAi involves the expression of long hairpin RNA (hpRNA). This method is facilitated by DNA constructs that... or multiple target sequences are concatenated. hpRNA has been extensively used in plant research and ...unique features, such as inducible expression of the hpRNA and/or fusing your gene target sequence to a fluorescent...include pGAPi, which allows easy creation of long hpRNA with Gateway-based cloning and straightforward positive...2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro RNA...hairpin RNA The greatest limitations of the long hpRNA approach are thought to be off-target effects and...efficiency. To address these shortcomings, both short hpRNAs (shRNAs) and artificial micro RNAs (amiRNAs) are...