Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 19 results for: SCFV

Showing: 1 - 19 of 19 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... all scFvs.   What are scFvs used for? scFvs are used in many of the ways antibodies are. scFvs are ...proteins. So let’s get acquainted with scFvs. What is an scFv? scFVs are a type of recombinant antibody. ...Advantages of scFvs: scFv’s advantages are rooted in their size. Being smaller means scFvs are easier and...what about single chain fragment variables (scFvs)? scFvs weren’t developed for another decade when in...oligomerization of the scFv. While you might assume that the variable domains of an scFv would mirror that ...configurations can generate functional scFvs; however, some individual scFvs performing better in one configuration...the IgG antibody and scFv.   What are the advantages and disadvantages of a scFv vs. a monoclonal antibody...

  2. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...variable fragment (ScFv) Figure 2: Comparison between the IgG antibody and scFv. Because the entire... details on ScFvs in this article: Antibodies 101: Single Chain Fragment Variables (scFvs). Nanobodies...regions alone can be generated as a fusion protein. An ScFv is made up of the variable regions of the heavy ...also plasmid-derived. However, a disadvantage to ScFv is their lower affinity for their target protein...plasmids that express antibodies, nanobodies, and ScFvs, as well as tools for developing and producing plasmid-based...Crivianu-Gaita V, Thompson M (2016) Aptamers, antibody scFv, and antibody Fab’ fragments: An overview and comparison...Wang S (2013) Engineering production of functional scFv antibody in E. coli by co-expressing the molecule...

  3. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...fragment (scFv), grafting the binding region of a FLAG-binding scFv onto a well-folded scFv scaffold to...complementarity determining region of an anti-FLAG scFv and a stable scFv scaffold. (b) Schematic of anti-FLAG-FB-GFP...

  4. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    Published
    Oct. 10, 2019, 12:41 p.m.
    ...Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) are fused to mature fluorophores...HA frankenbody, the Stasevich lab tested several scFvs scaffolds previously proven to fold and function...bind specific epitopes (Ewert et al., 2004). This scFv hybrid provides the probe structure and stability...fused a fluorescent protein to the hybrid anti-HA scFvs. The addition of the fluorescent protein allows ...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...version is needed.  Single-chain fragment variables (scFvs) are ~27kD engineered fusion proteins similar to... Minibodies are created by binding together two scFvs with a CH3 linker, resulting in an ~50kD protein...protein. Minibodies are bispecific, meaning that each scFv can target a different antigen; binding of each ...Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction to Antibodies Additional...

  6. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ... region (more on when that might be later). Find scFv plasmids at Addgene Depending on how an antibody...product called a single-chain Fragment Variable (scFv). Size: ~25 kDa.   Figure 2: Comparison...Introduction to AntibodiesSingle Chain Fragment Variables (scFvs)Avoiding the Mouse on Mouse Mess in IHC Resources...

  7. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ... a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential remedy for this...Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant antibodies...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...recruit GFP fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the SunTag...SunTag sites on the nascent polypeptide recruit scFV-GFP, generating green puncta that colocalize with the...

  10. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...screened antibody candidates using a synthetic human scFV library and characterized them by ELISA, homogeneous...synthetases.     Figure 2: Specific binding of scFVs to cognate aaRS antigens as measured by suspension...

  11. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...epitope GCN4. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed from a separate plasmid...

  12. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...antibodies and had begun developing Fab fragments and scFvs. But the potential for an even smaller, single-domain...

  14. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you ...

  17. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...enable many future variations using nanobodies or scFvs for other targets or could be used for applications...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...are recognized and thus fluorescently labeled by scFv antibodies fused to sfGFP. This system amplifies... to a scaffold containing epitopes recognized by scFV antibodies fused to VP64 transcriptional activation...
Showing: 1 - 19 of 19 results