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We narrowed to 63 results for: SHR

Showing: 1 - 20 of 63 results

  1. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...types, while shRNA and siRNA are synthetic constructs introduced through external methods. shRNA processing...plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The shRNA sequence is...BioRender.com.   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important... specificity. Using your shRNA plasmids One of the most common uses of shRNAs is to generate stable knockout...experiment. For this blog, we’ll be focusing on shRNA, one of the most popular choices for gene silencing... such as: Micro-RNA (miRNA) Short hairpin RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting ... is transcribed to produce hairpin shRNA structures that are processed by a ribonuclease called Dicer....

  2. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...variable is the shRNA. Therefore the empty control vector would be Plasmid A sans shRNA, or backbone Y ...Experiment: Knockdown the expression of Gene X using shRNA expressed from a plasmid   Figure 1: Expression ...a single experiment wherein Plasmid A (encoding shRNA against human Gene X in backbone Y) was transfected... this result one could simply conclude that the shRNA didn’t work, as the expression level of Gene X in...plasmid delivered into the cells successfully? Is the shRNA target correct? Is the purified plasmid DNA viable... in the experiment above, Plasmid A contains an shRNA that targets human Gene X and human cells are being...non-targeting control in this experiment could be an shRNA—in the same backbone as Plasmid A—that does not ...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...inducible gene overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion...vectors can regulate gene expression by delivering shRNA or miRNA. The ever expanding CRISPR field makes ...in mammalian cells.  Learn more about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-...empty backbones) here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has...Genetic Perturbation Platform, to produce their shRNA library to support functional investigations of ...sequence that can be removed and replaced with an shRNA sequence of your choice. More information and a ...

  4. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...lentiviral vector that contains a scrambled shRNA. This scrambled shRNA is used as a negative control for mammalian...expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini’s lab is a 3rd...mammalian RNAi experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector...transfer plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene...particles. It’s capable of inducible expression of shRNA in target cells by the addition of tetracycline/...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ... address these shortcomings, both short hpRNAs (shRNAs) and artificial micro RNAs (amiRNAs) are frequently...precursors of microRNAs found in the genome, whereas shRNAs are exogenously derived. Importantly, instead of...single ~21-nt interfering RNA is produced using shRNA/amiRNA. Although sh- and amiRNAs are likely more...Arabidopsis species, respectively. Short hairpin RNA (shRNA) is conceptually similar to the microRNA precursor...research community. Fortunately, many tools for shRNA in mammals are available at Addgene. Identifying...

  6. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...each insert.  Encodes gRNA/shRNA A plasmid with one or more gRNA or shRNA sequences cloned into it. gRNAs...location, while shRNAs are used to knockdown expression of mRNAs with complementarity to the shRNA sequence....contained within your insert (or targeted by a gRNA/shRNA) and select the species that the gene or insert ...

  7. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...thanks to our guest bloggers Shravanti Surest from Iowa State University. Shravanti Suresh is a PhD candidate...This post was contributed by Shravanti Suresh from Iowa State University. Since its appearance, SARS-...developing countries. You can follow her on Twitter @shra_krishna.       References Azhar, M., Phutela, R., Ansari...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression...constitutive or inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene...deletion, (3) simultaneous expression of cDNAs and shRNAs with a reporter gene, or (4) multiple sgRNAs with...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...several options to allow you to do exactly that: shRNAs are short RNA sequences which target complementary...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...the viral vector for your gene of interest – an shRNA sequence specific to your gene’s mRNA or a sgRNA...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...dozens of different mushroom varieties alone. At least I could tell that they were mushrooms. In some cases...and are especially abundant in neuronal synapses. shRNA silencing of one circular RNA they have identified...

  13. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...an RNAP II promoter, whereas small RNAs (such as shRNA) are transcribed from the RNAP III promoters. This...expression in plants. H1 small RNA expression shRNA From the human polymerase III RNA promoter Constitutive...expression in neuronal cells. U6 small RNA expression shRNA From the human U6 small nuclear promoter Constitutive...

  14. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...Methods Based on CRISPR/Cas by Shravanti Suresh Guest blogger Shravanti Suresh who studies CRISPR at Iowa...

  16. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...recombination to turn on or off the expression of shRNA. This system requires less work than using established...technical issues associated with high levels of shRNA expression. The capability to turn RUSH and CRUSH...the induction of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre...

  17. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...: it’s derived from the small (19 kDa) luminous shrimp (Oplophorus gracilirostris) luciferase and engineered...cruciata) Natural 562 64 Luciferin ATP No Luminous shrimp(Oplophorus gracilirostris) Natural 455 19 Coelenterazine...

  19. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... for: a wide range of DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all .... This system could be used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13...

  20. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...which is really important to her. Addgenie Shreya Vedantam with our resident dinosaur! Read her Instagram...Instagram feature here. One of our QC Scientists, Shreya Vedantam, left the bench not by choice, but by ...
Showing: 1 - 20 of 63 results