Skip to main content

We narrowed to 5 results for: TagRFP-T

Showing: 1 - 5 of 5 results
  1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...that placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T fluorescence intensity...FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence intensity due to interactions with...membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the...
  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein such as iFP1.4...#49004) to the spectra of both mCherry and TagRFP.  Here, the TagRFP spectrum is shown in the darker colors...peak of the TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission ... emission. For this filter set, we would expect TagRFP to give a brighter signal than mCherry. In general...shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like ...
  3. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...better understand T cell initiation in the adaptive immune response. It was known that T cells were able... fast T cells could jump from one antigen presenting cell to another. Indeed, we now know that T cells...constructs in the immune lineage. By specifically labeling T cells, B cells, and antigen presenting cells, scientists...answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the sites of infection...dendritic cells migrate to lymph nodes to activate T cells (Celli et al 2008, Kitano et al. 2016, Cavanagh...like EGFP Yellow-orange fluorescent proteins like TagRFP, tdTomato, DsRed, the mKate series, or tdKatushka2...example, have been successfully used to study B and T cell dynamics within the germinal center of murine...
  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...their emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...can fold in <10min at 37°C, mCherry takes ~15min, TagRFP ~100min and DsRed ~10hours. Temperature: FPs maturation...
Showing: 1 - 5 of 5 results