Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 11 results for: TagRFP-T

Showing: 1 - 11 of 11 results

  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...#49004) to the spectra of both mCherry and TagRFP.  Here, the TagRFP spectrum is shown in the darker colors...peak of the TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission ...or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein such as iFP1.4... emission. For this filter set, we would expect TagRFP to give a brighter signal than mCherry. In general...shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like ...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EEA1 GFP Silvia Corvera 42635 TagRFP-T-EEA1 Early endosomes EEA1 TagRFP-T Silvia Corvera 38770 pEF.myc.... pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Early...pTag-RFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc Recycling...pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc Recycling...

  4. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...better understand T cell initiation in the adaptive immune response. It was known that T cells were able... fast T cells could jump from one antigen presenting cell to another. Indeed, we now know that T cells...like EGFP Yellow-orange fluorescent proteins like TagRFP, tdTomato, DsRed, the mKate series, or tdKatushka2...constructs in the immune lineage. By specifically labeling T cells, B cells, and antigen presenting cells, scientists...answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the sites of infection...dendritic cells migrate to lymph nodes to activate T cells (Celli et al 2008, Kitano et al. 2016, Cavanagh...example, have been successfully used to study B and T cell dynamics within the germinal center of murine...

  5. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...can fold in <10min at 37°C, mCherry takes ~15min, TagRFP ~100min and DsRed ~10hours. Temperature: FPs maturation...their emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...is set as 1. Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pyogenes tagRFP Ebert pL-CRISPR.SFFV.tRFP 57826 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes tagRFP Ebert...Mammalian/Lentiviral BsmBI yes, cut S. pyogenes tagRFP Ebert pFGC-pcoCas9 52256 Plant AscI, PacI, SbfI.... All components are then assembled into a single T-DNA binary vector of choice through Gateway recombination...57824 Mammalian/Lentiviral BsmBI none S. pyogenes tagRFP657 Ebert pL-CRISPR.SFFV.GFP 57827 Mammalian/Lentiviral...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ... Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9...Content last reviewed 20 June 2025. References Das, A. T., Tenenbaum, L., & Berkhout, B. (2016). Tet-On Systems...window) Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral...Urlinger, S., Baron, U., Thellmann, M., Hasan, M. T., Bujard, H., & Hillen, W. (2000). Exploring the sequence...X., Vink, M., Klaver, B., Berkhout, B., & Das, A. T. (2006). Optimization of the Tet-On system for regulated...

  9. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...-35 mEGFP NA Cytoplasm 101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2 Centrioles 101782 LAMP1-mEGFP...protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T AICSDP-28 mTagRFP-T Alpha-tubulin Microtubules 101786 ST6GAL1...Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T Nucleophosmin Nucleolus (granular component...AAVS1-mTagRFPT-CAAX AICSDP-42 mTagRFPT CAAX domain of K-Ras Plasma Membrane 114403 LMNB1-mTagRFP-T AICSDP...References Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann...AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP NA Cytoplasm 114405...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... of the toolkit in action, see Cermak T, et al. 2017. Cermak T, et al. Plant Cell. 2017. PubMed PMID:...extra-chromosomal telomeric DNA), which accumulates when T-loops (secondary telomeric structures) are deleted...emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...(nm) Brightness pKa Maturation Structure Plasmids T-sapphire 399 511 26 4.9 1.3 hr Prone to dimerization... - Mammalian Expression TagRFP675 598 675 4 5 Prone to dimerization pTagRFP675-N1 - Mammalian Expression...Mammalian Expression TagRFP657 611 657 0.34 5.3 27 min Prone to dimerization pTagRFP657-N1 - Mammalian Expression...Expression pTagRFP657-C1 - Mammalian Expression pFA6a-link-yoTagRFP657-Kan - Yeast Expression smURFP +...Mammalian Expression PATagRFP 562 595 25 5.3 75 min Prone to dimerization pBAD/HisD-PATagRFP - Bacterial Expression...Plasmids rsTagRFP 440 / 567 585 / 585 0.02 / 4.0 6.6 43 min Prone to dimerization pBAD/HisD-rsTagRFP - Bacterial...Expression pBAD/HisD-TagRFP675 - Bacterial Expression mNeptune 600 650 13 5.4 35 min Prone to dimerization pcDNA3...
Showing: 1 - 11 of 11 results