Skip to main content

We narrowed to 8 results for: TagRFP-T

Showing: 1 - 8 of 8 results
  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein such as iFP1.4...#49004) to the spectra of both mCherry and TagRFP.  Here, the TagRFP spectrum is shown in the darker colors...peak of the TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission ... emission. For this filter set, we would expect TagRFP to give a brighter signal than mCherry. In general...shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like ...
  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...EEA1 EGFP Silvia Corvera 42635 TagRFP-T-EEA1 Endosomes (early) EEA1 TagRFP-T Silvia Corvera 38770 pEF.myc.ER-E2...pTag-RFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes...pTag-RFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc Endosomes...pTag-RFP-C-h-Rab4a-c-Myc Endosomes (recycling) Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc Endosomes...
  3. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...better understand T cell initiation in the adaptive immune response. It was known that T cells were able... fast T cells could jump from one antigen presenting cell to another. Indeed, we now know that T cells...constructs in the immune lineage. By specifically labeling T cells, B cells, and antigen presenting cells, scientists...answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the sites of infection...dendritic cells migrate to lymph nodes to activate T cells (Celli et al 2008, Kitano et al. 2016, Cavanagh...like EGFP Yellow-orange fluorescent proteins like TagRFP, tdTomato, DsRed, the mKate series, or tdKatushka2...example, have been successfully used to study B and T cell dynamics within the germinal center of murine...
  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...their emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...can fold in <10min at 37°C, mCherry takes ~15min, TagRFP ~100min and DsRed ~10hours. Temperature: FPs maturation...
  5. Tetracycline Inducible Expression

    Type
    Collection
    ... Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9...Plasmids Stem Cell Research Plasmids References Das, A. T., Tenenbaum, L., & Berkhout, B. (2016). Tet-On Systems...window) Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew, R., & Schiedlmeier, B. (2011). Retroviral...Urlinger, S., Baron, U., Thellmann, M., Hasan, M. T., Bujard, H., & Hillen, W. (2000). Exploring the sequence...X., Vink, M., Klaver, B., Berkhout, B., & Das, A. T. (2006). Optimization of the Tet-On system for regulated...
  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...ipUSEPR-TR657 ATM TagRFP CMV Ataxia telangiectasia Sika Zheng 228918 sg_Atm_i5-ipUSEPR-TR657 ATM TagRFP CMV Ataxia...226380 pcDNA3.1(-).TagRFP.T-2A-Tau2N4R-WPRE MAPT TagRFP, T2A CMV, T7 Parkinson's, FTD Bradley Hyman 226381...226381 pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's, FTD Bradley Hyman...pcDNA3.1(-).TagRFP.T-2A-V5-8D2Q.Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's, FTD Bradley Hyman...pcDNA3.1(-).TagRFP.T-2A-V5-20D3Q.Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's, FTD Bradley Hyman...pcDNA3.1(-).TagRFP.T-2A-V5-8A2R.Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's, FTD Bradley Hyman...pcDNA3.1(-).TagRFP.T-2A-V5-20A3R.Tau2N4R-WPRE MAPT TagRFP, T2A, V5 CMV, T7 Parkinson's, FTD Bradley Hyman...
Showing: 1 - 8 of 8 results