...evokes action potentials (APs) in patched cells expressing wild type CoChR, these APs are absent in soCoChR-expressing... little signal detected along the neurite (Figure 1.) Moreover, the group was able to demonstrate that...Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of TagRFP-T ...

... cerevisiae (Tuller et. al., 2010). Figure 1: An overview of peptide synthesis. A ribosome interacts...doi.org/10.1016/j.tibtech.2004.04.006 Hausser J, Syed AP, Bilen B, Zavolan M (2013) Analysis of CDS-located...McClain WH (2000) The G·U wobble base pair. EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001...example, Henry Lester’s lab optimized the C. elegans GluCl ion channel genes for expression in mammalian cells...2003) Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically...

...horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but for simplicity...alternative detection methods.      Figure 1: The four types of ELISA are direct, indirect, sandwich...conjugated antibody is not readily available. Table 1: Overview of the advantages and disadvantages of each...empowered to choose the best ELISA for your needs. Good luck and happy binding! More resources on the Addgene...

...phosphorylated DNA termini to 3 ́-OH DNA termini (1-4). In vitro, ligases (notably T4 DNA ligase) are ... enzymology of DNA repair enzymes.     References 1. Shuman, Stewart, and Christopher D. Lima. "The polynucleotide...thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G., et al. "Cloning...Veronique Bailly, and Walter G. Verly. "Nicks 3′ or 5′ to AP sites or to mispaired bases, and one-nucleotide gaps...PubMed Central PMCID: PMC1978145. 27. Greenough, Lucia, et al. "Adapting capillary gel electrophoresis ...

...in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while...INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins forming the QCascade...Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-Guided...stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this lethality...