Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 18 results for: basic aav ram vector

Showing: 1 - 18 of 18 results

  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...terrains. They are, in fact, a choice delivery vector from basic science research all the way to clinical gene...specifically AAV, are also being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low...We offer a variety of viral vectors, viral services and even a Viral Vectors eBook to get you started. ...on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift mutations...of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application, our...interest, turning expression off permanently. Viral vectors can also be used to express proteins. Viral expression...

  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ... parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find AAVs for your experiment...making the AAVs you need for your experiment:  the packaging plasmid which contains the AAV structural...capsid (cap) gene. In wild-type AAV, these two genes are found between the AAV genome’s two inverted terminal...68 play a key role in AAV genome replication. Replication initiates from the AAV genome ITRs on the transfer...clearly defined yet. Now you’ve got a basic understanding of the AAV RepCap plasmid! Check out this blog..., Strohl WR (2017) Adeno-Associated Virus (AAV) as a Vector for Gene Therapy. BioDrugs 31:317–334 . https... for Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development...

  3. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...-stranded AAV (ssAAV) and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of ... got the basics of AAVs down, you’re ready to take the next step – selecting your AAV and AAV components...about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All ... lot of AAV serotypes, many of which are available in rAAVs!   Figure 1: Diagram of AAV components...chemogenetics experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo ...speak about AAVs, they are actually referring to rAAVs. ITRs and serotypes The names of AAVs contain two... self-complementary AAV (scAAV) may be an option (McCarty et al., 2001). This AAV is designed so that ...

  4. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...Made in Heaven: CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery...Repair Addgenie Chari Cortez explains some of the basic science behind homology directed repair (HDR) and...101: Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired...the clever ways to successfully combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once...post, Addgenie Melina Fan guides us through the basics of the validation process for CRISPR mediated genome...Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ... work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel Hill explain some of the molecular...

  5. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...choosing AAV or lentiviral vectors in our previous blog posts.  Viral vector injection Viral vectors allow...expression vector and the vector particles are delivered into the brain region of interest. AAV or lentivirus...lentivirus?  Both AAV and lentiviral vectors are used for intracerebral injections. AAV particles are smaller...lentiviral vectors are superior packaging vectors for larger genes - they can hold up to 8 kb, while AAV can ...viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors Resources...possible outcomes. Typically, AAVs have to be minimum around 1012-1013 vg/ml (vector genomes per milliliter)...using viral vectors By modulating neuronal gene expression and activity, you can use viral vectors to study...

  6. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...micrograph of an Addgene AAV viral vector preparation after negative staining. Empty vector particles can be ...get more reliable AAV titering? For our viral service, we currently titrate AAV vector preparations by ...by using the universal AAV Reference Standard Material (AAV SFM), which is an AAV sample that has been ... qPCR strategy for titrating AAV vectors." Medical science monitor basic research19 (2013): 187. PubMed...infectivity of wild-type AAV as benchmark for infectivity of recombinant AAV vectors." Gene therapy 17.7 (...Considerations When Using AAVs Addgene's AAV Quality Control Methods Check Out Our Viral Vector Featured Topic Page...to titer AAV! The point is there are many ways to quantify the composition of a viral vector solution,...

  7. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...split vector strategies plus one additional split vector strategy for increasing the size of the AAV donor...homology directed repairs (HDR). Split AAV vector approaches Split vector approaches increase the size of the...greater understanding of basic AAV biology, however, would help improve split vector strategies and lead to...time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging capacity.... So how do you fit a big gene into a tiny vector like AAV? By breaking the transgene into smaller pieces...into AAV First, some history. In 2008, the Auricchio lab decided to solve the issue of “tiny vector, big...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... come back here once you are up to speed on the basics of the technology. In this blog, we will highlight...kit has a focus on infrastructure with shuttle vectors, several delivery systems which can be engineered...engineered into the TUs, and even viral gene silencing vectors. The Plant Parts III kit has a focus on transformation... Modular Cloning Kit which it builds upon (its ‘basics’ MoClo plant kit buddy). It is important to understand...too. The AspFlex Kit was developed to study the basic biology of and gene regulation in the opportunistic...and to compare different Cas enzymes in all-in-one AAV delivery. While Fragmid developers were specifically...Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo...

  9. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing... 2025, we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene...today. “I generally refer people to Addgene for the basics of molecular biology and cloning, since they have...2017, we had distributed 1,000 ready-to-use viral vector preps. Today, that number has grown to over 114,600...research community once more. Our ready-to-use viral vectors were useful for researchers wanting high quality...standardized agreements and electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled...

  10. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...the promoter in the AAV construct. Stereotaxic surgery  As mentioned above, AAV opsin constructs are...spatial precision (I’m not going to discuss the basic biology of optogenetics so if you are not familiar...his optogenetic constructs available through the vector cores of various universities as well as Addgene...transfer agreement, and list of vendors (such as the vector core at the University of North Carolina) that ...is local injection of an adeno-associated viral (AAV) construct using stereotaxic surgery (see videos .... The Deisseroth lab has developed many of these AAV constructs with various promoters and other genetic...However, the length of promoter that can be used in an AAV construct is limited to only a few kb so attaining...

  11. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...has led to their application for basic and translational research.  Basic research applications of scFvs...size also allows for their delivery by viral vectors like AAV, which could be useful for delivering scFvs...domains of the selected scFvs are inserted into a vector encoding the antibody constant domain scaffold ...doi.org/10.1038/nbt.1791  Deal CE, Balazs AB (2015) Vectored antibody gene delivery for the prevention or treatment...2017) Complementarity determining regions and frameworks contribute to the disulfide bond independent ...

  12. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...terminator will suffice. Many commercial expression vectors use double terminators to reduce unwanted translation...as well studied as prokaryotic termination, the basic processes for eukaryotic termination are understood... handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID: 10074136...effects of various terminators in the context of AAV transgene expression levels. Transcription Termination...formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ. An Internal...

  13. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important...Having seen CRISPR’s success in basic research, researchers are eager to apply it in a clinical setting...culture, but Ran et al. have shown that CRISPR and AAV can be used together for postnatal genome editing...facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause obvious germline modification...with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively...reading frame. gRNAs bind in intronic regions, so low-frequency indels will not cause frameshifts. Figure...approximately 1 in 5000 males. DMD is caused primarily by frameshift mutations in dystrophin, a protein essential ...

  14. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... existing transposon and vector systems and creating an all-synthetic vector that included only the elements... into a destination vector in a predefined order. Finally, the destination vectors are used with Multisite... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke et al...step, compatible Level 0 vectors are directionally assembled into a Level 1 vector creating a single transcriptional...of interest into the pCoofy vectors, select the appropriate pair of vector and gene primers from Table...homology between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence ...

  15. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ...expression vectors with various promoters suitable for in vivo expression, and/or produce vectors to make...prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen et al 2013) allow...donor vectors containing mAID and either Neo or Hygro resistance markers with CRISPR/Cas vectors targeting...which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids optimized... 18165312 CIDAR MoClo parts kit Synthetic and basic biologists alike often need to test multiple expression... interest and cloned into provided destination vectors using low-cost, one-pot golden gate cloning that...

  16. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through...needed (for knock in models). CRISPR mouse model basics To create a mouse model, the gRNA, Cas9, and donor...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...and opened a sequence file. Then I saw it, the chromatogram showing the telltale signs of a mutation introduced... find that many, if not all, the mice carry a frameshift mutation. For knock in with a donor oligonucleotide...

  17. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...to Keep a Lab Notebook for Bioinformatics AnalysesAAV Vector Quality Control: Going the Extra Mile with...: documentation. We oriented ourselves with the basic concepts and vocabulary of Nextflow so that we could...solutions for each in the form of one-off scripts or programs, our goal was to find a suitable software tool...Nextflow’s design, we were free to use multiple programming languages (Python, Perl, Java, R, etc.) in our...workflow without having to translate or convert programs to a uniform language.  The modern architecture...provided by a container is sequestered from other programs running on a computer, so it safely operates in...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...MXS-chaining kit contains 5 empty chaining vectors plus a destination vector and a collection of building blocks...populations move and interact over time. A Ramm lab plasmid with an N1-vector-compatible LOC8R sequence is also...inducible gene expression Brilliant technologies adopt basic biological concepts and employ them in creative ...
Showing: 1 - 18 of 18 results