We narrowed to 3 results for: be3 encoding gene

...original BE3 and HF-BE3 using a gRNA with high off-target cutting, and they found that HF-BE3 showed 37...activity than BE3  85172  pBK-EQR-BE3  EQR-Cas9 (NGAG)  Lower off-target activity than BE3  85173  pBK-VRER-BE3...  pJL-SaBE3  SaCas9 (NNGRRT)    85170  pJL-SaKKH-BE3  SaCas9 (NNGRRT)    85171  pBK-VQR-BE3  VQR-Cas9... pBK-VRER-BE3  VRER-Cas9 (NGCG)    85174  pBK-YE1-BE3  SpCas9 (NGG)  Editing window ~2 nt  85175  ...  pBK-EE-BE3  SpCas9 (NGG)  Editing window ~2 nt  85176  pBK-YE2-BE3  SpCas9 (NGG)  Editing window ~... ~2 nt  85177  pBK-YEE-BE3  SpCas9 (NGG)  Lower on-target activity than BE3; editing window ~1-2 nt ...understanding of B. subtilis gene functions and interactions. The single-gene deletion library is derived...

...transcription from the accessory plasmid (AP) encoding phage gene III. The accessory plasmid library contained... xCas9 3.7 fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM...(MP6) permits quick, large-scale mutation of the gene of interest. They created a selection phage (SP)... a library PAM, it would induce transcription of gene III, permitting phage propagation and keeping that...PAM editing efficiency over that of SpCas9-BE3 (37 vs 28%). Cytidine base editing at NGT, NGC, GAA, and... 13 (2.1) 38 (4.5) 7.2 (5.2) xCas9(3.7)-BE3 37 (1.3) 16 (3.5) 6.2 (13) 24 (9.5) 10 ...evolution to generate and select for Cas9 mutants with PAM flexibility. In this system, the mutagenesis plasmid...

...CRISPRi system, the Laub lab repressed ctrA, a gene encoding an essential cell cycle regulator. The lab expressed...demethylation Optogenetic repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic...2019. Fusing epigenetic modifiers such as TET1 with inactive Cas enzymes can alter gene expression via...optogenetic toolkit that controls repression of gene expression in Saccharomyces cerevisiae. The system consists... enters the nucleus where dCas9-Mxi1 can repress gene expression. In the dark, the NLS is inaccessible...off-target DNA editing The original cytosine base editor, BE3, induces a low frequency of genome-wide off-target...dilutions of CRISPRi in Caulobacter cells for ctrA gene expression using various dCas9. Image adapted from...