We narrowed to 4 results for: blasticidin resistance
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TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m.... Conferring Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd HeLa, ...plasmid contains a cassette that will make cells resistant to a toxin), whereas negative selection would ...reagent). Cells should die within 3-5 days and resistant colonies appear in about 10-14 days depending ...confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin is typically...Backbone Read More: Plasmids 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All... antibiotic resistance...
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Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....determined by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In their Nature...16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m...., or other. Bacterial Resistance Enter bacterial antibiotic resistance encoded in the plasmid that...glycerol stock. Choose from: ampicillin, apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m.... lentiviral backbones expressing blasticidin or puromycin resistance genes. It’s that simple! Good luck...