We narrowed to 2 results for: dcas9 vp64 blast

1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ...dead Cas9 (dCas9) based activators (such as dCas9-VP64) or repressors (dCas9-KRAB or dCas9-SRDX) can also... W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of...

2. 27 Hot Plasmids from 2016

   Type

   Blog Post

   Published

   Dec. 22, 2016, 3:03 p.m.

   ...target genes (20-40x greater than standard dCas9-VP64 activators) when introduced into mammalian cells....creating a new dCas9-VPR expression plasmid that uses the piggybac transposase to integrate dCas9-VPR into ... is genomic integration of dCas9-VPR and controlled expression of the dCas9-VPR transgene by doxycycline...methylation by fusing catalytically inactive Cas9 (dCas9) with the catalytic domain from DNA methyltransferase...Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3, pTALYM4 ... activator developed by the Church lab, termed “dCas9-VPR”, which facilitates robust activation of target...activate target genes, the relatively large size of dCas9-VPR limits one’s ability to package it into lentivirus...