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We narrowed to 5 results for: doxycycline inducible human factors

Showing: 1 - 5 of 5 results

  1. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...gene expression by administering an inducing agent like doxycycline (Favre et al., 2002). They are useful...experiment. Some inducible constructs can exhibit leaky expression in the absence of the inducing drug, so be...Correlates with Long-Term Doxycycline-Regulated Transgene Expression in Nonhuman Primates after Intramuscular...know what expression pattern you need, and the factors in your system that can affect expression, before...before starting the selection process. Factors to think about are (1) your experimental conditions and measurements...in a mouse may not work as well in a rat or non-human primate. Likewise, in vitro efficacy doesn’t always...it can be ubiquitous, cell-type selective, or inducible.  Ubiquitous promoters  These often-strong promoters...

  2. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... repressor in hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids include...Catalysis by TEV protease allows a transcription factor to enter the nucleus and modulate transcription... and results in uniform NB biotinylation. Once induced, the NBs could be easily purified by one-step immobilized... new multiplexed CRISPRi and CRISPRa system for human pluripotent stem cells By Jennifer Tsang CRISPR ...design and delivery. To overcome these variations in human pluripotent stem cells(hPSCs), Lindy Barrett’s lab...page.  PAC-MAN (prophylactic antiviral CRISPR in human cells) is a CRISPR-Cas13 based antiviral method ...influenza A virus. It was demonstrated to work in human lung epithelial cells. Find the plasmids at Addgene...

  3. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...transfer vector with an inducible promoter (see below) should be used. Inducible lentiviral transfer vectors...separate genus of the Retroviridae family including human immunodeficiency virus type-1 (HIV-1), can infect...) especially if you are working on HIV positive human cell from the clinic. The 2nd generation is similar...if you are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not...with the oncogenic virus, or that the accessory factors in HIV positive cells could make the virus replication...possibility that mutation or recombination with human retroviruses could lead to RCL. Regarding transduction...mutagenesis: Replication-incompetent lentiviruses with human tropism can still infect the researcher and insert...

  4. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_403. The chosen attR destination vector...desired constructs: lentiviral expression of the human KRAS gene in mammalian cells. STEP 1: Generate an...different ways to generate our desired entry clone - human KRAS flanked by attL sites.  Method A: recombination...experiment. This choice will depend on a number of factors, like your organism, desired expression level, ...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer...and rtTA. The cells were pulsed with 2µg/ml of Doxycycline for 2 hours, and imaged on the Keyence BZ-9000...protein may upregulate an alternative pathway or induce other compensatory mechanisms. How can protein ...mitochondria (binding FRB), allowing for a rapid and inducible inactivation. Find the Robinson plasmids or the...this system by engineering cells that lack release factor one (RF-1; Bacterial strain EcAR7) and minimizing... Rinehart lab synthesized the activated form of human mitogen-activated ERK activating kinase 1 (MEK1)...plasmids from Scot Wolfe's lab. These include Tet-inducible CRISPR activators and repressor plasmids. (Kearns...
Showing: 1 - 5 of 5 results