...EGFP) [1, 2] or even form obligate tetramers (i.e. DsRed) [3]. This is a problem for fusion proteins. One...as EGFP, but not for obligate oligomers such as DsRed or high affinity FPs such as TagRFP. Problem 3 can...References 1. Snapp, E.L., et al., Formation of stacked ER cisternae by low affinity protein interactions. J...

...C-terminus of DsRed.T3. See Table 1 for a summary of Rosella’s excitation and emission spectra. DsRed is the ... phosphatase. Normally PHO8 is synthesized at the ER, delivered to the vacuole via the secretory pathway...proteins: a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic...spectra. Component Excitation Emission pH Range DsRed.T3 488, 543*, 568 nm 587 nm ~4.9 - 9 SEP ...

...dual colour-emission biosensor Rosella consists of DsRed connected to a pH-sensitive variant of GFP (SEP)...Figure 1). The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while SEP fluoresces...environments that inactivate SEP and leave only DsRed to fluoresce (1). With variants that can be targeted... previously unsuspected genes with known roles in ER-associated degradation (ERAD) that positively regulate...