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We narrowed to 5 results for: fire lab vector kit

Showing: 1 - 5 of 5 results
  1. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...requested! Fire Lab C. Elegans Kit - Our first popular "kit," Andrew Fire's set of vectors for C. elegans...popular kit of the year was the Golden Gate TALEN Kit, which continues to be our most requested kit of the...expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier Trono's lab are our...cells. Plasmids from Shinya Yamanaka's lab and James Thomson's lab were the most frequently requested. Ubiquitin...first distributed plasmid and was created in the lab of my thesis advisor, Dr. Bruce Spiegelman. It was...expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with...technology has been used and modified by so many labs and for so many different purposes that we had to...
  2. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...selection marker (Kim et al., 2014). Meanwhile Andy Fire’s lab used oligo-mediated knock-in of a dominant mutation...SapTrap CRISPR/Cas Toolkit and SapTrap-SEC kit for C. elegans genome engineering have also facilitated CRISPR... C. elegans. These modular toolkits build single plasmid targeting vectors that encode the gRNA transcript...The first approach – co-CRISPR from Craig Mello’s lab – used inactivation of the unc-22 gene as their selection...past few years:  Repair template design The Meyer lab meticulously characterized several aspects of editing...repair templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification ...2020). Together, these improvements from the Mello lab resulted in strikingly high editing efficiencies,...
  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...mini-transposon vector for bacterial mutagenesis or gene targeting Victor de Lorenzo's lab has engineered... existing transposon and vector systems and creating an all-synthetic vector that included only the elements...elements needed for function. The lab validated this vector, called pBAM1, by conducting random mutagenesis...”) by Margaret Robinson’s lab, and more recently used by Stephen Royle’s lab, acutely depleted proteins... into a destination vector in a predefined order. Finally, the destination vectors are used with Multisite... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...RNAi transgenic mice The Jackson-Grusby lab has designed two vectors for use in conditional and reversible...
  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... New kit for editing dicotyledonous plants The Stuttmann lab generated the vectors in this kit to make...Design Automation Research lab Moduclar Cloning (CIDAR MoClo) Parts Kit. This Kit consists of a variety of...plasmids in this kit are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being...these receptors. Bryan Roth's lab has a newly available PRESTO-TANGO kit which allows researchers to interrogate...Examples of FusX-Compatible Destination Vectors Available at Addgene: Lab Plasmids Dan Carlson RCIscript-GoldyTALEN...both laboratory and prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen...expression vectors with various promoters suitable for in vivo expression, and/or produce vectors to make...
  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    .... The MXS-chaining kit contains 5 empty chaining vectors plus a destination vector and a collection of... time. A Ramm lab plasmid with an N1-vector-compatible LOC8R sequence is also available, N1-LOC8R.  ...Cohen lab wished to improve on the Arch indicator and their collaborators in Robert Campbell’s lab carried...targeted fluorescent molecules. The Vale lab and the Weissman lab have teamed up and advanced technology...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...
Showing: 1 - 5 of 5 results