...better probe the functions of the human c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified...noticed that Myc1-9E10 and the other C-terminal antibodies only recognized the human c-Myc protein and ...linear sequences within the middle of the human c-Myc protein or the C-terminus, Evan, et al. isolated six ...bound to the C-terminal peptide. One of these C-terminal antibodies was Myc1-9E10 (aka anti-c-Myc [9E10])....when Evan’s team generated their Myc antibodies. At first, anti-c-Myc [9E10] was just one of several new...Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610–...Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...

...the C-terminal region of the human proto-oncogene c-Myc. Since the generation of the first anti-c-Myc ...on the Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here...light-gated potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available! Improved voltage...doi.org/10.1101/2021.09.17.460684.)   Myc-tag antibody anti-c-Myc [9E10] now available! by: Ashley Waldron...Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. Dec;5(...to guess that the Myc-tag is one of them (and not just because of the title!) The Myc-tag is a string of...), the Myc tag has become one of the most commonly used protein tags - a quick search for ‘Myc tag’ in...

...enhances iPSC generation as does replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying ...addition of an shRNA for P53 and replacing C-MYC with L-MYC in the episomal reprogramming cocktail by ... reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation of clinical grade hiPSCs... that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method...therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs) now provides an opportunity to develop...for retention and replication of the vectors in human cells [8]. The EBNA-1 protein recognizes OriP and...pCXWB-EBNA-1 lacks oriP and is unable to replicate in human cells, thus expressing additional EBNA-1 transiently...

...monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived...negatively charged and 1 positively charged amino acids. Myc tags are frequently used for western blot, flow cytometry... However, protein purification is not ideal with Myc, as low pH conditions are required during the purification...affect protein function. Fun fact: The antibody to Myc was developed in 1985 and is still the most prevalent...Addgene has you covered with monoclonal antibodies to Myc for all of these species. HA The HA tag is a 9 amino... amino acid peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively...availability Plasmids 101: Protein Tags Antibodies: Of Myc and Men    ...

...cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with ...genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can...for identification of essential MYC binding sites in a wide range of human cells.  A new pooled library ...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells, a cell type that has been...library from Junjie Chen's lab targets human genes known or suspected to be involved in DNA damage response ... system is capable of robust expression of high G+C content genes, as validated through monitoring the...

...fluorescent proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also created the Monoclonal Antibody... by the National Institute for Child Health and Human Development (NICHD) in 1986, to enable basic research...monoclonal antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha...single sample; 3) producing recombinant, partially humanized rabbit antibodies that exhibit increased avidity...

...four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to revert...additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is one of the reprogramming factors.... Patsch, C., et al., Generation of vascular endothelial and smooth muscle cells from human pluripotent...easily isolated from donors. Apart from fibroblasts, human keratinocytes from hair pluck, peripheral blood ...types have been successfully differentiated from human iPSCs. Below is a list of some of these cell types... Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 2007. 131(...Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007. 318(5858): p. 1917...

...Zhi Ying Chen, Robert C. Robbins, Mark A. Kay, Michael T. Longaker, and Joseph C. Wu. "A nonviral minicircle... viral particles that express oncogenes, such as Myc. Viral vectors also have the largest genomic footprint...transgenes is a concern as well, and reactivation of Myc or other oncogenes after differentiation has been... of these functional tests aren’t possible with human iPSCs, the the ability to generate teratomas is ...Robert Lanza, and Kwang-Soo Kim. "Generation of Human Induced Pluripotent Stem Cells by Direct Delivery...and Mahendra S. Rao. "A Review of the Methods for Human iPSC Derivation." Methods in Molecular Biology Pluripotent...minicircle vector for deriving human iPS cells." Nature Methods 7, no. 3 (2010): 197-99. PubMed PMID: 20139967...

...allows users to easily create C-terminal tag fusions of endogenous genes in human cells. To use CRISPaint, ... T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann...Natsume, Toyoaki, et al. “Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short...PCR or by evaluating visual phenotypes. Improving C. elegans fluorescent protein tagging with SapTrap ...called SapTrap to improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists a convenient one...protein tagging systems For those who do not work with C. elegans, several groups have designed modular toolkits...plasmid containing the homology arms and an N- or C-terminal tag. The two PCR reactions are then mixed...

... of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse fibroblasts...from the inner cell mass (ICM) of a 5-6 day old human blastocyst [6]. They are isolated mostly from abnormal... cell niches” found in many organs in the adult human body. These generate specialized cells for their... Self-renewal High Low once isolated from the human body Usage Universal. Can generate all adult cell...Thomson labs showed the successful generation of human iPSCs from dermal fibroblasts via retroviral and...., Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med, 2004. 350(13): p. 1353...Turetsky, T., et al., Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after...

...validated their specificity in mice, non-human primates, and human tissue. Figure 6: Enhancer-based...or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin resistance gene makes stable...recombinant antibodies against three of the six human glypican proteins (GPC1, GPC3, and GPC4). All IPI...known regulators at the FOS gene promoter and the MYC locus (Figure 5). Figure 5: TurboCas protein...expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for single action potential-evoked...integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger, L., Lim, J., Hiraizumi, M., … Abudayyeh... Cenik, B. K., Aoi, Y., Iwanaszko, M., Howard, B. C., Morgan, M. A., Andersen, G. D., Bartom, E. T., &...

...lentiviral sgRNA expression yielded editing of 80–90% in human cell lines and primary cells. Although cell entry...Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B...Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted genome... for non-invasive systemic gene delivery in non-human primate (NHP) brains.  Systemic gene delivery capsids...fluorescent reporters in green monkeys and cultured human neurons, with broader neuronal expression than AAV9...functional intravenous gene transfer throughout the non-human primate brain. Nature Nanotechnology, 18, 1241–1251...mChe), with co-incubation with assist peptide (AP). C) Workflow for Cas-RNP-PAGE editing of primary T cells...

...protocol starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the...the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody candidate...due to camelid high body temperature (37.2 - 39.2°C). The Seeger lab found a way around this by using ...Enzyme Variants. In: Methods in Molecular Biology. Humana Press, pp 133–148. https://doi.org/10.1007/978-...

...j.cell.2021.09.018 Choi, J., Chen, W., Suiter, C. C., Lee, C., Chardon, F. M., Yang, W., Leith, A., Daza,...expression ~ ~ ~ ~ ✓   ✓ SV40 and c-Myc nuclear localization sequences Improve translocation..., J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A.,...pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database. Previously...reverse transcriptase with a sequence optimized for human codons; additional nuclear localization sequences...of heteroduplex     ✓ ✓ ~ ~ ~ Human codon optimization Improve prime editor expression...Leukemia Virus (M-MLV) reverse transcriptase to the C-terminus of Cas9 H840A nickase (PE1). But innovations...

..., the tool is available for human and mouse genomes only and deals with C-terminus epitope tagging. If...(3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely failed. If C-terminus...Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. ...antibody for each target protein. To study 20,000 human proteins, this would be time-consuming, expensive...Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent tagging...your crRNAs using other web-based design tools (for C-terminus tagging, pick a crRNA that cuts 8-15 bp downstream...insertion site (within ~8-15 bp from the stop codon for C-terminus tagging) GC content of the crRNA (aim for...

...of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic...elucidate the mechanism by which SOX2 acts to reprogram human fibroblasts into induced pluripotent stem cells....article have been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation...Mammalian Expression Construct SpCas9 Inhibition (Human Cells) acrIIA1 Listeria monocytogenes  pRAU168...fluorophores with nonoverlapping emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, ...coselection, analogous to methods previously used in C. elegans, targets the sodium/potassium pump ATP1A1... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...

...physiologically similar to humans than many other model organisms, they are often used to study human diseases. Diseases...counterpart in humans (Liu et al., 2017) . Thus yeast can be used to study the molecular basis of human diseases...They share about 70% of their genes with humans and 85% of human genes associated with a disease have a ...completely sequenced and over 60% of human genes have an ortholog in C. elegans making it ideal to study ...PMCID: PMC5525645. Mello, Craig C., et al. "Efficient gene transfer in C. elegans: extrachromosomal maintenance... 190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression ...the globe. These organisms share many genes with humans, are easily maintained in the lab, and have short...

... 79628 (C) Cv (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635 NS3 C 79637...79637 NS2B C 79636 NS4A N 79640 NS4B C 79639 NS5 C 79632 (N) and 79631 (C) PrM N and C   Detecting... both human and fly versions. Arnold, et al. Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral...   Human kinase domain constructs for automated bacterial expression The misregulation of human kinases...Lab recently compiled the Human Kinase Domain Constructs Kit, a library of human kinase domain constructs...tagged BioID2 for N-terminal fusions and Myc tagged BioID2 for C-terminal fusions. Kim, et al. Mol Biol...gRNAs targeting a total of 20,121 genes within the human genome (the identity of the genes and target sequences...

... Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates target DNA ...activation, the c-terminal cas9 fragment was fused with FK 506 binding protein 12 (Cas9(C)-FKBP-2xNLS) and...export sequence. Treatment with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and...thermophilus which have been used for gene editing in human cells without cross-talk in cognate sgRNA binding...understanding the spatio-temporal regulation of human genes. The plasmids are all available at Addgene...These three enzymes were chosen because they cut human and mouse coding regions infrequently; any MluI,...while there are many types of epitope tags (eg. HA, MYC, Flag), they can be deleterious and cause aberrant...

...different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry... expression systems that can be added to the N or C terminus of your protein of interest. Many of these...Gene Expression Protocols (Vol. 205, pp. 99–118). Humana Press. https://doi.org/10.1385/1-59259-301-1:99...