Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 256 results for: human dat

Showing: 1 - 20 of 256 results

  1. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...Consortium Healthy human cells Imaging (IHC, ICC, IF, etc.)  Data repository From the Human BioMolecular...Any Data repository   YCharOS Human proteins Immunoblot (western blot), IP, and IF. Data repository...others) Data repository From the Human Cell Differentiation Molecules group Addgene Data Hub Any...imaging Data repository See blog post mAb3D Atlas Mouse and human brain  IHC Data repository... Any Data repository From the Frederick National Library for Cancer Research Human Luekocyte... include validation data CiteAb Any Any Search engine May include validation data  Biocompare...websites that can help: Antibody data repositories, which share validation data, and antibody search engines...

  2. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...conditions commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76...Researcher Perspectives on Data Sharing in Deep Brain Stimulation. Frontiers in Human Neuroscience, 14(December...Vector-Mediated Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089...Adeno-Associated Virus Serotype 9 (AAV9) Preparations. Human Gene Therapy Methods, 30(6), 214–225. https://doi.org... AAV Data with Addgene's New AAV Data HubCelebrate Open Data Day with Addgene’s AAV Data Hub!Data Freedom...asking scientists what data would improve our AAV Data Hub.   The Addgene Data Hub is here to help!  ...through DataCite before being made publicly available through the Data Hub. These minimum data standards...

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...the Human Leucocyte Differentiation Antigens (HCDM) workshop approach, which focuses on validating antibodies...antibody validation efforts and information provided by external organizations like the Human Cell Differentiation...Bradding, P. (2019). Validation of antibodies for the specific detection of human TRPA1. Scientific Reports...antibodies against markers of the human blood leukocyte populations, as well as a pragmatic approach to...characterizing cell surface molecules expressed on human blood leukocytes, including antibodies against these...Roach, K. M. (2021). TGFβ1 induces resistance of human lung myofibroblasts to cell death via down-regulation...target type. To start the validation process, one must first confirm that a candidate antibody can detect the...

  4. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...translate AID to yeast, as well as mammalian and human cell lines (Nishimura et al., 2009, Natsume et al... your protein of interest to create conditional human cell lines. Lastly, the Kanemaki lab demonstrated...conversion in the target sites adjacent to an NG PAM in human cells. The increased versatility of the engineered... for degradation using AID Article contributed by Alyssa Cecchetelli Auxin Inducible Degradation (AID)...publicly available metagenomic data including the Tara Oceans Project datasets. This study demonstrates the...available metagenomic datasets with biophysical studies and the potential of mining datasets for research and...interest fused to a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1...

  5. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...confident in your data. This is a good option for applications like immunohistochemistry on human tissues, when...favorite protein in human tissues. You know that there is a wealth of transcriptomic data from this sample...your gene of interest across a set of human tissues.  From this data, you see that there is high RNA expression... major takeaway from these recommendations is: validate, validate, validate. This doesn’t necessarily ...extensive validation experiments for every antibody that you receive; citing existing validation data can be...extra validation experiments yourself.  Goal of Antibody Validation  As you assess existing data or plan...appropriately validated so that you can have confidence in your results. But what is appropriately validated? This...

  6. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... reveals specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: ...Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells." Nature Biotechnology (2016). PubMed PMID... RA, van der Oost J, Backofen R, Koonin EV. An updated evolutionary classification of CRISPR-Cas systems...Pages Check Out MpAgo from the Doudna Lab Find Validated gRNAs ...

  7. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... beta-3 Human, Mouse IgG1 Rabbit Anti-Integrin alpha L [IPI-TS2/4] Integrin alpha-L Human IgG1 Rabbit...alpha-L Human IgG1 Rabbit Anti-Integrin alpha M [IPI-M1/70] Integrin alpha-M Mouse, Human IgG1 Rabbit...Integrin alpha-M Human IgG1 Rabbit Anti-Integrin alpha M [IPI-CBRM1/29] Integrin alpha-M Human IgG1 Rabbit...alpha-M Mouse, Human IgG2a Rat Anti-Integrin alpha M [IPI-CBRM1/5] Integrin alpha-M Human IgG2a Mouse...Integrin alpha-M Human IgG2a Mouse Anti-Integrin alpha M [IPI-LM2/1] Integrin alpha-M Human IgG2a Mouse...environments. Found on nearly every cell in the human body, integrins are key to a diverse array of processes..., and others are rat or mouse IgG2a. Additional human IgG1s are also available on demand. The 11 IPI synthetic...

  8. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...stability in human plasma The last trick GeoCas9 has up its sleeve is its increased longevity in human serum....Figure 2: Human plasma longevity of SpCas9 vs GeoCas9. SpCas9 or GeoCas9 RNPs were incubated in human plasma... in diluted human plasma. GeoCas9 remains active after being incubated in up to 30% human plasma. ...increased thermostability also had a longer lifetime in human blood. To test if this was also true for GeoCas9...al incubated SpCas9 and GeoCas9 RNPs in diluted human plasma for 8 hours at 37°C. Substrate was then added... reaction conditions. Finally, its longevity in human serum may allow for more efficient genome editing...most excited about? Thermostability? Longevity in human plasma? Something we haven’t thought of yet? Let...

  9. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...similarities with humans. However, rats have some key differences in physiology, intelligence, and human correspondence...corresponds with human diseases and conditions. For example, the rat is frequently used to study human cardiovascular...sequencing and concluded that nearly all disease-linked human genes have counterparts in the rat (Gibbs, et al..., studying the axolotl may reveal the potential humans have for a similar degree of regeneration.  In ...phenotypic changes that are similar to those observed in humans. Thus, understanding their life cycle and processes...regenerative abilities is expected to shed light on human aging pathological developments (Vieira et al., ...patterns are conserved, making them great models for human disease research. One of their most attractive features...

  10. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...far enough along, scientifically, to be doing in humans; We have not had a broad, public conversation or... scientifically unproven technologies to edit a human embryo, or have a woman carry an edited embryo to...international moratorium on utilizing CRISPR/Cas9 in humans. This topic was to be revisited at meetings when...social and ethical issues of genome editing and human reproduction, the relevant document for the question... research, animal models, and several stages of human clinical trials. This model also demonstrates that...always been people who unethically experiment on humans, and they are not remembered kindly. However, despite...was recognized as an unethical experimentation on human subjects, utilizing a scientific technique not proven...

  11. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...viral proteins in human cells and used these viral “bait” proteins to fish for human “prey” proteins that...rely on the help of human proteins in their lifecycle, identifying these potential human helpers of SARS-...Leary (UCSF). Our approach is to discover key human proteins that can be targeted (hopefully by existing...BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in which we expressed 26 of ... with the virus. As a result, we identified 332 human proteins that the virus interacts with in cells....therapeutic monkeywrench into the virus’ hijacking of human cells. Even better, more than half the drugs hitting... of understanding how SARS-CoV-2 interacts with human proteins. For example, we used HEK293T cells in ...

  12. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...understand how each human TF can contribute to differentiation, the Zhang lab transduced human embryonic stem...accompanying expression atlas, including over 3,500 human TF isoforms, to help researchers understand this... library The transcription factor library The human genome encodes approximately 1,800 TFs, with >3,...packaged in lentiviral particles and used to transduce human embryonic stem cells. Transduced stem cells were...to that of reference cell types generated by the human fetal expression atlas. Computational analysis was...tool to be used in this capacity. Similar to the Human TFome Library, the MORF library can be used with...library amplification Zhang Lab CRISPR Plasmids The Human TFome Library  ...

  13. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...time of sampling if available. STR profiling for human, mouse, and dog cell lines is included when available...Using the data provided by the researcher, Cellosaurus was able to both update their database and sent ...Rigor and reproducibility of scientific data depends on validated reagents along with clear documentation...called Cellosaurus, a growing cell line information database describing nearly 145,000 cell lines. Cellosaurus...2 research, or adenovirus packaging cell lines dataset collections.  Depending on available information...cell line you’ve used? Submit your manuscript or data to Cellosaurus via [email protected] community...sent the updated information to the cell line distributor. A success all around! If you’re excited about ...

  14. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...science,” said Guillaume Bourque, a Professor in Human Genetics at McGill University. “There is a need ... new, centralized databases and centers to support the sharing of materials and data associated with COVID...into COVID-19 (like here at Addgene!), and new databases are supporting their access to useful resources...internationally COVID-19 Resources Canada is a database that provides information about reagents, funding...co-founded the COVID-19 Resources Canada site. To date, the portal has recruited 3,218 Canadian volunteers...supports collaborations at Rutgers, but also lists data and experimental resources that are useful to any...for COVID-19 research in their field including datasets and platforms for discussing new developments,...

  15. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...have a human counterpart, enabling study of human-relevant pathways (Liu et al., 2017). Beyond human relevance...problematic for human administration. Thus, the production of glycoproteins for human therapies, such ...have its genome fully sequenced There are several human disease models established in yeast, including a...antibodies, is possible in K. phaffii since it produces ‘human-like’ glycosylated proteins. Peroxisome studies..., Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression modules. Biomedical...sequestering certain enzymes and performing specific oxidative reactions. They are found in all eukaryotic cells...tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation K. phaffii...

  16. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...proteins are necessary, HEKs are human in origin, so they eliminate non-human PTMs which is a concern with...al., Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293...Ever wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly... What the HEK is wrong with it?   All cultured human cell lines are susceptible to contamination by mycoplasma.... Are HEKs for you?   If you are looking for a human cell line model, HEKs might be a great option! Especially...include receptor biology, ion channels, or involve human protein preparations with PTMs. Why the HEK not?...293 cells are tumorigenic, which makes them a candidate cell line model for cancer-specific research. ...

  17. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... synthetic promoter AAVs in mice, non-human primates and humans. Nature Neuroscience, 22, 1345-1356. https...for targeting the same cell class in humans (R=0.67).  A database of the microscopy images of all AAVs...synthetic promoters performance in mouse, monkey, and human retinas Next, the 113 AAV promoters that were active...retinas were injected into macaques and postmortem human retinas. About 25% of these AAV synthetic promoters...promoters successfully targeted cells in monkey and human retinas. By comparing the performance of promoters...targeting the same cell class in NHPs (R=0.38) and humans (R=0.32), but the odds of that promoter targeting...chances of a promoter expressing in primate and human retinas but not in mice was low (R=0.12), suggesting...

  18. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...of the Human Biomolecular Atlas Program (HuBMAP) antibody metadata standards. And the dataset is rich...biology enthusiasts. This dataset currently includes 1049 community-validated reagents from 20 contributors...Zenodo dataset.     Figure 2: Screenshot showing reagent entries with associated metadata adapted... and the database. They particularly encourage people to upload and share their negative data. After all...into conversations with other users. The entire dataset can be downloaded from Zenodo, including the reagent...rich. Experimental data includes reagent type, target species, target name, host organism, isotype, clone...documentation that agrees or disagrees with the recommendation. The goal is to easily share and find what ...

  19. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...cell lines from humans and other species. By accurately compiling genome-wide datasets, which are often...relevant metadata and the screen results. Screenshots updated 2024. Importantly, metadata for each...CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited in...published data by biomedical researchers and ready access to stably archived large-scale datasets by computational...allows users to query screen data without the need to analyze raw sequence data.  This format is inherently...your CRISPR screen data in ORCS  Researchers who wish to have their CRISPR screen data included in the BioGRID...through the supplemental files to see the actual data. Now you’re wondering if your favorite gene is included...

  20. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...understand the basis for cardiovascular and metabolic human diseases. They do that by studying patients to uncover...studying those variants in model systems: either human cells or mice. In a recent issue of Cell Stem Cell...efficient tool for doing that disease modeling work in human pluripotent stem cells: a transcription activator-like...their TALEN kit to quickly and efficiently generate human stem cells edited to carry mutant versions of 15...various reasons it is not nearly as efficient in human stem cells. What genome editing tools allow you ...implications of this kit in terms of understanding human disease? Musunuru: The power here is that you could.... "A TALEN genome-editing system for generating human stem cell-based disease models." Cell Stem Cell....
Showing: 1 - 20 of 256 results