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We narrowed to 2 results for: human h2b gfp

Showing: 1 - 2 of 2 results
  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...mutate in the genome) and an out-of-frame H2B (histone)-GFP reporter into the genome of mammalian cells...puromycin resistance gene linked to an in-frame H2B-GFP. This visual read out allows for easy screening... the supplied donor plasmid will express GFP. If there’s GFP expression, presumably the endogenous target...targeted the gene NFE2L2, the Madison lab found that H2B-GFP expressing cells exhibited a 2-4 fold increase ...RFP to GFP expressing cells provides a real-time read out of Cas9 activity.  By using the RFP:GFP ratio... can easily and seamlessly remove the integrated GFP reporter leaving you with just your desired genomic...target a specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter ...
  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...both the GFP and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins...Compartment mCherry-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B Mitochondria mito-BFP...GTPase in division) GFP-Mff (outer membrane protein) Late Endosome mCherry-Rab7A GFP-Rab7A     ...2015). He has also constructed a library of eGFP-tagged human ORF clones to allow testing and comparison...protein Knock-off, is made up of three components: a GFP protein that binds to the coding region of a reporter...reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter...thermophilus which have been used for gene editing in human cells without cross-talk in cognate sgRNA binding...
Showing: 1 - 2 of 2 results