...can be expressed from lentiCas9-Blast. Purpose of LentiCas9-blast LentiCas9-blast is a Cas9 expression... were (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based...express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9-expressing...lentiviruses. This means that if you received lentiCas9-blast lentivirus from Addgene, you could use it in...your own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing... causes growth rate variability when using lentiCas9-blast to generate Cas9-expressing cells. To ensure...

...with lentiCas9-blast. A549 cells were transduced (MOI = 37) and selected with 1 µg/mL blasticidin for ... transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells were then ...Cas9 expression in A549 cells transduced with lentiCas9-blast. Anti-Cas9 Western blotting was performed on... PMID: 18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang...then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed...

...lentiviral backbone that must be used with lentiCas9-Blast or with cell lines already expressing Cas9....